Strain Data Sheet

RBRC02338

Strain Information

Image
BRC No.RBRC02338
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-p130Cas<tm2.1Homy>
Former Common nameCas-FL conditional KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Hiroaki Honda, Hiroshima University in 2005.
Strain descriptionBcar1 (p130Cas) is an adaptor molecule on growth factor receptor and integrin activation functions as regulator of cell motility and invasion. This conditional knockout mice have a loxP-flanked Bcar1 allele. Conditional Bcar1 deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenancebackcross to C57BL/6N (Heterozygote x C57BL/6NCrlCrlj)
References
Skeletal muscle adaptation in response to mechanical stress in p130cas-/- mice.
Akimoto T, Okuhira K, Aizawa K, Wada S, Honda H, Fukubayashi T, Ushida T
Am. J. Physiol. Cell Physiol., 304(6):C541-7 (2013). 23325412

p130Cas, Crk-associated substrate, plays important roles in osteoclastic bone resorption.
Nagai Y, Osawa K, Fukushima H, Tamura Y, Aoki K, Ohya K, Yasuda H, Hikiji H, Takahashi M, Seta Y, Seo S, Kurokawa M, Kato S, Honda H, Nakamura I, Maki K, Jimi E
J. Bone Miner. Res., 28(12):2449-62 (2013). 23526406

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bcar1breast cancer anti-estrogen resistance 18Bcar1targeted mutation 2.1, Hiroaki Honda
Frtyeast FRT (flippase recombination target) site8Frt
loxPphage P1 loxP8loxP
loxPphage P1 loxP8loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • muscle phenotype(MP:0005369)
  • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, rat Cas cDNA, SV40 poly A, yeast FRT site, mouse Cas genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHiroaki Honda (Tokyo Women's Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    Akimoto T, Okuhira K, Aizawa K, Wada S, Honda H, Fukubayashi T, Ushida T.
    Skeletal muscle adaptation in response to mechanical stress in p130cas-/- mice.
    Am J Physiol Cell Physiol 304(6) C541-7(2013) 23325412
    Miyazaki T, Zhao Z, Ichihara Y, Yoshino D, Imamura T, Sawada K, Hayano S, Kamioka H, Mori S, Hirata H, Araki K, Kawauchi K, Shigemoto K, Tanaka S, Bonewald LF, Honda H, Shinohara M, Nagao M, Ogata T, Harada I, Sawada Y.
    Mechanical regulation of bone homeostasis through p130Cas-mediated alleviation of NF-κB activity.
    Sci Adv 5(9) eaau7802(2019) 31579816