Strain Information | |
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Image | |
BRC No. | RBRC02338 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129P2-p130Cas<tm2.1Homy> |
Former Common name | Cas-FL conditional KO |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Dr. Hiroaki Honda, Hiroshima University in 2005. |
Strain description | Bcar1 (p130Cas) is an adaptor molecule on growth factor receptor and integrin activation functions as regulator of cell motility and invasion. This conditional knockout mice have a loxP-flanked Bcar1 allele. Conditional Bcar1 deficient mice can be generated by crossing with tissue-specific Cre mice. |
Colony maintenance | backcross to C57BL/6N (Heterozygote x C57BL/6NCrlCrlj) |
References | Skeletal muscle adaptation in response to mechanical stress in p130cas-/- mice. Akimoto T, Okuhira K, Aizawa K, Wada S, Honda H, Fukubayashi T, Ushida T Am. J. Physiol. Cell Physiol., 304(6):C541-7 (2013). 23325412p130Cas, Crk-associated substrate, plays important roles in osteoclastic bone resorption. Nagai Y, Osawa K, Fukushima H, Tamura Y, Aoki K, Ohya K, Yasuda H, Hikiji H, Takahashi M, Seta Y, Seo S, Kurokawa M, Kato S, Honda H, Nakamura I, Maki K, Jimi E J. Bone Miner. Res., 28(12):2449-62 (2013). 23526406 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Bcar1 | breast cancer anti-estrogen resistance 1 | 8 | Bcar1 | targeted mutation 2.1, Hiroaki Honda | |||
Frt | yeast FRT (flippase recombination target) site | 8 | Frt | ||||
loxP | phage P1 loxP | 8 | loxP | ||||
loxP | phage P1 loxP | 8 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, rat Cas cDNA, SV40 poly A, yeast FRT site, mouse Cas genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Hiroaki Honda (Tokyo Women's Medical University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Akimoto T, Okuhira K, Aizawa K, Wada S, Honda H, Fukubayashi T, Ushida T. Skeletal muscle adaptation in response to mechanical stress in p130cas-/- mice. Am J Physiol Cell Physiol 304(6) C541-7(2013) 23325412 |
Miyazaki T, Zhao Z, Ichihara Y, Yoshino D, Imamura T, Sawada K, Hayano S, Kamioka H, Mori S, Hirata H, Araki K, Kawauchi K, Shigemoto K, Tanaka S, Bonewald LF, Honda H, Shinohara M, Nagao M, Ogata T, Harada I, Sawada Y. Mechanical regulation of bone homeostasis through p130Cas-mediated alleviation of NF-κB activity. Sci Adv 5(9) eaau7802(2019) 31579816 |