Strain Data Sheet


Strain Information

BRC No.RBRC02322
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Cmah<tm1Ykoz>
Former Common nameCmah null mouse, KZO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Hiromu Takematsu and Yasunori Kozutumi, Graduate School of Biostudies, Kyoto University in 2000. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionCmah gene knockout mice. A neo cassette was inerted into the second coding exon. This mouse is deficient for the biosynthesis of N-glycolylneuramic acid, one of the major species of sialic acid. Due to the deficiency, sialic acid species of this mouse is almost 100% N-acetylneuraminic acid, which is the precursor of N-glycolylneuraminic acid in the biosynthetic pathway. Since CMAH is one of rare gene inactivated in human but retained in the other homonids such as chimpanzee. Thus, this mouse could by utilized to mimic human in terms of the sialic acid species expression.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesMol. Cell. BIol., 27, 3008-3022 (2007). 17296732

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cmahcytidine monophospho-N-acetylneuraminic acid hydroxylase13Cmah<tm1Ykoz>targeted mutation 1, Yasunori KozutsumiCMP-NeuAc hydroxylase

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)13neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse PGK promoter, E.coli reomycin resistance gene, mouse Cmah genomic DNA
Research applicationMetabolism Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 27, 3008-3022 (2007).For use of the BIOLOGICAL RESOURCE by a for—profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHiromu Takematsu (Fujita Health University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data