Strain Data Sheet

RBRC02322

Strain Information

Image
BRC No.RBRC02322
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Cmah<tm1Ykoz>
Former Common nameCmah null mouse, KZO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Hiromu Takematsu and Yasunori Kozutumi, Graduate School of Biostudies, Kyoto University in 2000. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionCmah gene knockout mice. A neo cassette was inerted into the second coding exon. This mouse is deficient for the biosynthesis of N-glycolylneuramic acid, one of the major species of sialic acid. Due to the deficiency, sialic acid species of this mouse is almost 100% N-acetylneuraminic acid, which is the precursor of N-glycolylneuraminic acid in the biosynthetic pathway. Since CMAH is one of rare gene inactivated in human but retained in the other homonids such as chimpanzee. Thus, this mouse could by utilized to mimic human in terms of the sialic acid species expression.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Germinal center marker GL7 probes activation-dependent repression of N-glycolylneuraminic acid, a sialic acid species involved in the negative modulation of B-cell activation.
Naito Y, Takematsu H, Koyama S, Miyake S, Yamamoto H, Fujinawa R, Sugai M, Okuno Y, Tsujimoto G, Yamaji T, Hashimoto Y, Itohara S, Kawasaki T, Suzuki A, Kozutsumi Y
Mol. Cell. BIol., 27, 3008-3022 (2007). 17296732

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cmahcytidine monophospho-N-acetylneuraminic acid hydroxylase13Cmahtargeted mutation 1, Yasunori Kozutsumi
neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal B cell physiology(MP:0002459)

  • abnormal T cell physiology(MP:0002444)

  • abnormal humoral immune response(MP:0001800)

  • abnormal inner ear morphology(MP:0000026)

  • abnormal interleukin secretion(MP:0008568)
  • more 15 phenotypes
  • abnormal organ of Corti morphology(MP:0000042)

  • abnormal semicircular canal morphology(MP:0002428)

  • abnormal sensorimotor gating(MP:0002634)

  • abnormal startle reflex(MP:0001486)

  • cochlear outer hair cell degeneration(MP:0004404)

  • decreased interferon-gamma secretion(MP:0008567)

  • hematopoietic system phenotype(MP:0005397)

  • immune system phenotype(MP:0005387)

  • impaired coordination(MP:0001405)

  • impaired hearing(MP:0006325)

  • impaired wound healing(MP:0001792)

  • increased B cell proliferation(MP:0005154)

  • increased IgG1 level(MP:0008499)

  • increased vertical activity(MP:0002574)

  • premature death(MP:0002083)
  • Detailed phenotype data

    Ordering Information

    Donor DNAmouse PGK promoter, E.coli reomycin resistance gene, mouse Cmah genomic DNA
    Research applicationMetabolism Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 27, 3008-3022 (2007). For use of the BIOLOGICAL RESOURCE by a for—profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorHiromu Takematsu (Fujita Health University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data