Strain Data Sheet

RBRC02317

Strain Information

Image
BRC No.RBRC02317
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Pde10a2<tm1(tTA)Yok>
Former Common namePDE10A2-tTA
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Yokoi, Columbia University in 2002.
Strain descriptionPde10a2 tTA knock-in mice. A tTA gene was knocked into PDE10A2 gene locus, and the PDE10A2 gene is knocked out. The homozygotes and heterozygotes can be used as PDE10A2-knockout mice, and as a tool for the specific expression in striatal projection neurons, respectively.
Colony maintenance
References
Inducible expression of retrograde transynaptic genetic tracer in mice.
Hiromi Sano, Yumiko Nagai, Mineto Yokoi
Genetics, 45, 123-128 (2007) 17299748

Striatal medium spiny neurons terminate in a distinct region in the lateral hypothalamic area and do not directly innervate orexin/hypocretin- or melanin-concentrating hormone-containing neurons.
Hiromi Sano, Mineto Yokoi
J. Neurosci., 27, 6948-6955 (2007) 17596443

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pde10a
MGI:1345143		phosphodiesterase 10A17Pde10a<tm1(tTA)Yok> targeted mutation 1, Yokoi Mineto
  • obsolete dyskinesia, limb and orofacial, infantile-onset(MedGEN)

  • striatal degeneration, autosomal dominant 2(MedGEN)
    • loxP phage P1 loxP17loxP
    tTA tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)17tTA

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal social investigation (MP:0001360)
    • Detailed phenotype data

    Ordering Information

    Donor DNAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain), Phage P1 loxP site, mouse Pde10a2 genomic DNA
    Research applicationCre/loxP system
    Neurobiology Research
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 27, 6948-6955 (2007). RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
    DepositorYokoi Mineto (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Suzuki E, Momiyama T.
    M1 muscarinic acetylcholine receptor-mediated inhibition of GABA release from striatal medium spiny neurons onto cholinergic interneurons.
    Eur J Neurosci 53(3) 796-813(2021) 33270289