Strain Data Sheet

RBRC02302

Strain Information

Image
BRC No.RBRC02302
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Lyzs<tm1(cre)Ifo>
Former Common nameLysM-Creノックインマウス (C57BL/6), B6.129P2-Lyzs<tm1(cre)Ifo>
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6NTac
Appearanceblack [a/a B/B C/C]
Strain developmentTechnical University of Munich・Irmgard Foersterらにより開発。秋田大学医学部（鈴木　聡来教授）を経由（2003年6月）して、慶應大学医学部に導入された。E14 ES細胞を用いて作出。RBRC02302：C57BL/6に数世代戻し交配されたものを、C57BL/6NTacに15代以上戻し交配したもの。RBRC02303：C57BL/6に数世代戻し交配されたものを、BALB/cAnNに15代以上戻し交配したもの。
Strain descriptionLysMcreマウスと呼ばれている。単球・マクロファージなどのmyeloid細胞に高発現するリソソーム酵素であるMリゾチームの染色体遺伝子座にCreをインフレームで挿入したノックインマウス。myeloid細胞特異的かつ効率的に遺伝子操作が可能なCreマウスである。C57BL/6背景 (RBRC02302) およびBALB/c背景 (RBRC02303) 。
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NTac]
References
Conditional gene targeting in macrophages and granulocytes using LysMcre mice.
Clausen B E, Burkhardt C, Reith W, Renkawitz R, Forster I
Transgenic Res., 8, 265-277 (1999) 10621974

Health Report

Examination Date / Room / Rack2024/06/10Room:3-5Rack:A
2024/03/11Room:3-5Rack:A
2023/12/11Room:3-5Rack:A
2023/09/11Room:3-5Rack:A
2023/06/13Room:3-5Rack:A
2023/03/13Room:3-5Rack:A
2022/12/12Room:3-5Rack:A

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site10Frt
  • familial visceral amyloidosis(MedGEN)
    • Lyz2lysozyme 210Lyz2targeted mutation 1, Irmgard Foerster
  • familial visceral amyloidosis(MedGEN)
    • crePhage P1 Cre recombinase10cre
  • familial visceral amyloidosis(MedGEN)
    • nlsSV40 large T antigen nuclear localization signal (NLS)10nls
  • familial visceral amyloidosis(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • enlarged spleen(MP:0000691)
    • Detailed phenotype data

    Ordering Information

    供与核酸Simian virus 40 Large T antigen nuclear localization signal (NLS), Phage P1 cre, yeast FRT (flipase recombination target) site, mouse lysozyme M genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    FLP/frt system
    Immunology and Inflammation Research
    提供条件条件を付加する。
    利用者は事前に開発者の許可を得る。
    開発者連絡先：Dr. Irmgard Foerster, Life and Medical Sciences (LIMES) Institute, University of Bonn E-mail: irmgard.foerster@uni-bonn.de.
    Depositor小安　重夫（慶応義塾大学）
    Strain Status生体のアイコン生体
    凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    生体マウスを1~3ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Sato H, Taketomi Y, Miki Y, Murase R, Yamamoto K, Murakami M.
    Secreted Phospholipase PLA2G2D Contributes to Metabolic Health by Mobilizing ω3 Polyunsaturated Fatty Acids in WAT.
    Cell Rep 31(5) 107579(2020) 32375030
    Akbari M, Honma K, Kimura D, Miyakoda M, Kimura K, Matsuyama T, Yui K.
    IRF4 in dendritic cells inhibits IL-12 production and controls Th1 immune responses against Leishmania major.
    J Immunol 192(5) 2271-9(2014) 24489086
    Mitsui K, Hishiyama S, Jain A, Kotoda Y, Abe M, Matsukawa T, Kotoda M.
    Role of macrophage autophagy in postoperative pain and inflammation in mice.
    J Neuroinflammation 20(1) 102(2023) 37131209
    Nakamura Y, Nakanishi T, Shimada H, Shimizu J, Aotani R, Maruyama S, Higuchi K, Okura T, Deguchi Y, Tamai I.
    Prostaglandin Transporter OATP2A1/SLCO2A1 Is Essential for Body Temperature Regulation during Fever.
    J Neurosci 38(24) 5584-5595(2018) 29899035