Strain Data Sheet

RBRC02302

Information1

Image
BRC No.RBRC02302
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Lyzs<tm1(cre)Ifo>
Former Common nameLysM-Cre knock-in mice (C57BL/6)
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6NTac
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Technical University of Munich, Irmgard Foerster. Deposited by Shigeo Koyasu, School of Medicine, Keio University through Akita University Graduate School of Medicine. E14 ES cells were used to generate the knock-in mice. The mice were crossed to C57BL/6 for several times, and then backcrossed to C57BL/6NTac and BALB/cAnN, respectively (RBRC02302 and RBRC02303).
Strain descriptionLyzs (Lyz2, lysozyme 2) Cre knock-in mice, known as the LysMcre mouse. A NLS-cre cDNA was inserted into the endogenous ATG start site of Lyz2 gene. LysMcre mice allow for both specific and highly efficient Cre-mediated deletion of loxP-flanked target genes in myeloid cells. C57BL/6 background (RBRC02302) and BALB/c background (RBRC02303).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NTac]
ReferencesTransgenic Res., 8, 265-277 (1999) 10621974

Health Report

Examination Date / Room / Rack2023/03/13Room:3-5Rack:A
2022/12/12Room:3-5Rack:A
2022/09/12Room:3-5Rack:A
2022/06/13Room:3-5Rack:A
2022/03/14Room:3-5Rack:A
2021/12/14Room:3-5Rack:A
2021/09/13Room:3-5Rack:A
2021/06/14Room:3-5Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lyzslysozyme10Lyzs<tm1(cre)Ifo>targeted mutation 1, Irmgard FoersterLC, Lys-cre, LysM Cre, lysM/cre, LysMCre, LysMcre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site10Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase10cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)10nls

Information2

Donor DNASimian virus 40 Large T antigen nuclear localization signal (NLS), Phage P1 cre, yeast FRT (flipase recombination target) site, mouse lysozyme M genomic DNA
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER, Dr. Irmgard Foerster, Life and Medical Sciences (LIMES) Institute, University of Bonn E-mail: irmgard.foerster@uni-bonn.de.
DepositorShigeo Koyasu (Keio University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Genetic Background
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Sato H, Taketomi Y, Miki Y, Murase R, Yamamoto K, Murakami M.
Secreted Phospholipase PLA2G2D Contributes to Metabolic Health by Mobilizing ω3 Polyunsaturated Fatty Acids in WAT.
Cell Rep 31(5) 107579(2020) 32375030

Akbari M, Honma K, Kimura D, Miyakoda M, Kimura K, Matsuyama T, Yui K.
IRF4 in dendritic cells inhibits IL-12 production and controls Th1 immune responses against Leishmania major.
J Immunol 192(5) 2271-9(2014) 24489086

Nakamura Y, Nakanishi T, Shimada H, Shimizu J, Aotani R, Maruyama S, Higuchi K, Okura T, Deguchi Y, Tamai I.
Prostaglandin Transporter OATP2A1/SLCO2A1 Is Essential for Body Temperature Regulation during Fever.
J Neurosci 38(24) 5584-5595(2018) 29899035