Strain Data Sheet

RBRC02300

Strain Information

Image
BRC No.RBRC02300
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Pten<tm2Mak>
Former Common namePten-flox mouse (C57BL/6)
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Tak W. Mak, Ontario Cancer Institute in 2001. Deposited by Dr. Shigeo Koyasu, Keio University School of Medicine. C57BL/6N background.
Strain descriptionPten is a lipid phosphatase associated with tumor suppression. This conditional knockout mice have a loxP-flanked Pten allele. Conditional Pten deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenancebackcross to C57BL/6 (Heterozygote x C57BL/6NTac)
References
T cell-specific loss of Pten leads to defects in central and peripheral tolerance.
Suzuki A, Yamaguchi M T, Ohteki T, Sasaki T, Kaisho T, Kimura Y, Yoshida R, Wakeham A, Higuchi T, Fukumoto M, Tsubata T, Ohashi P S, Koyasu S, Penninger J M, Nakano T, Mak T W
Immunity, 14, 523-534 (2001) 11371355

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ptenphosphatase and tensin homolog19Ptentargeted mutation 2, Tak W Mak
  • Cowden disease(DisGeNET)

  • Cowden syndrome 1(MedGEN)

  • endometrial carcinoma(DisGeNET)
    • more 8 Diseases
  • familial meningioma(MedGEN)

  • glioma susceptibility 2(MedGEN)

  • head and neck squamous cell carcinoma(DisGeNET)

  • Lhermitte-Duclos disease(DisGeNET)

  • macrocephaly-autism syndrome(DisGeNET, MedGEN)

  • melanoma(DisGeNET)

  • prostate cancer(DisGeNET, MedGEN)

  • PTEN hamartoma tumor syndrome(DisGeNET)
    • loxPphage P1 loxP19loxP
  • Cowden disease(DisGeNET)

  • Cowden syndrome 1(MedGEN)

  • endometrial carcinoma(DisGeNET)
    • more 8 Diseases
  • familial meningioma(MedGEN)

  • glioma susceptibility 2(MedGEN)

  • head and neck squamous cell carcinoma(DisGeNET)

  • Lhermitte-Duclos disease(DisGeNET)

  • macrocephaly-autism syndrome(DisGeNET, MedGEN)

  • melanoma(DisGeNET)

  • prostate cancer(DisGeNET, MedGEN)

  • PTEN hamartoma tumor syndrome(DisGeNET)
    • loxPphage P1 loxP19loxP
  • Cowden disease(DisGeNET)

  • Cowden syndrome 1(MedGEN)

  • endometrial carcinoma(DisGeNET)
    • more 8 Diseases
  • familial meningioma(MedGEN)

  • glioma susceptibility 2(MedGEN)

  • head and neck squamous cell carcinoma(DisGeNET)

  • Lhermitte-Duclos disease(DisGeNET)

  • macrocephaly-autism syndrome(DisGeNET, MedGEN)

  • melanoma(DisGeNET)

  • prostate cancer(DisGeNET, MedGEN)

  • PTEN hamartoma tumor syndrome(DisGeNET)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal Purkinje cell morphology(MP:0000877)

  • abnormal astrocyte physiology(MP:0008916)

  • abnormal behavior(MP:0004924)

  • abnormal bone marrow cavity morphology(MP:0000065)

  • abnormal bone structure(MP:0003795)
    • more 76 phenotypes
  • abnormal cartilage morphology(MP:0000163)

  • abnormal cerebellar foliation(MP:0000857)

  • abnormal cerebellar granule cell morphology(MP:0004098)

  • abnormal cerebellar molecular layer(MP:0000889)

  • abnormal cerebellum external granule cell layer morphology(MP:0000872)

  • abnormal cerebellum morphology(MP:0000849)

  • abnormal chondrocyte morphology(MP:0000166)

  • abnormal dentate gyrus morphology(MP:0000812)

  • abnormal epiphyseal plate morphology(MP:0006395)

  • abnormal glomerular capillary morphology(MP:0011320)

  • abnormal heart left ventricle morphology(MP:0003921)

  • abnormal heart morphology(MP:0000266)

  • abnormal hepatocyte morphology(MP:0000607)

  • abnormal hippocampus morphology(MP:0000807)

  • abnormal hippocampus pyramidal cell layer(MP:0008284)

  • abnormal kidney collecting duct morphology(MP:0004754)

  • abnormal liver morphology(MP:0000598)

  • abnormal long bone epiphysis morphology(MP:0000131)

  • abnormal long bone hypertrophic chondrocyte zone(MP:0000165)

  • abnormal long bone morphology(MP:0003723)

  • abnormal renal glomerulus morphology(MP:0005325)

  • abnormal spike wave discharge(MP:0008840)

  • abnormal tumor susceptibility(MP:0002166)

  • abnormal urinary bladder urothelium morphology(MP:0000540)

  • abnormal urothelium morphology(MP:0003630)

  • ataxia(MP:0001393)

  • cardiac hypertrophy(MP:0001625)

  • decreased Purkinje cell number(MP:0000880)

  • decreased body size(MP:0001265)

  • decreased cardiac muscle contractility(MP:0005140)

  • decreased hippocampus pyramidal cell number(MP:0009971)

  • ectopic cartilage(MP:0003869)

  • ectopic ureteric bud(MP:0010980)

  • endometrium hyperplasia(MP:0009092)

  • enlarged heart(MP:0000274)

  • enlarged liver(MP:0000599)

  • epidermal hyperplasia(MP:0001222)

  • gliosis(MP:0002183)

  • hair follicle outer root sheath hyperplasia(MP:0010688)

  • hepatic steatosis(MP:0002628)

  • hindlimb paralysis(MP:0000755)

  • hippocampal neuron degeneration(MP:0000811)

  • hydrocephaly(MP:0001891)

  • hydronephrosis(MP:0000519)

  • impaired branching involved in ureteric bud morphogenesis(MP:0004936)

  • increased body length(MP:0001257)

  • increased bone mineral density(MP:0000062)

  • increased brain size(MP:0005238)

  • increased circulating alanine transaminase level(MP:0002941)

  • increased circulating alkaline phosphatase level(MP:0002968)

  • increased hamartoma incidence(MP:0010306)

  • increased hepatocellular carcinoma incidence(MP:0003331)

  • increased hepatocyte proliferation(MP:0003893)

  • increased incidence of tumors by chemical induction(MP:0004499)

  • increased liver adenoma incidence(MP:0003324)

  • increased liver cholesterol level(MP:0010027)

  • increased liver triglyceride level(MP:0009355)

  • increased liver weight(MP:0002981)

  • increased lymphoma incidence(MP:0012431)

  • increased myocardial fiber size(MP:0004564)

  • increased prostate gland adenocarcinoma incidence(MP:0009220)

  • increased prostate intraepithelial neoplasia incidence(MP:0009219)

  • increased urinary bladder transitional cell carcinoma incidence(MP:0009551)

  • kyphosis(MP:0000160)

  • lethargy(MP:0005202)

  • liver fibrosis(MP:0003333)

  • liver inflammation(MP:0001860)

  • long tail(MP:0002758)

  • megacephaly(MP:0000434)

  • neuron hypertrophy(MP:0005603)

  • pale liver(MP:0000603)

  • postnatal lethality, complete penetrance(MP:0011085)

  • premature death(MP:0002083)

  • ruffled hair(MP:0000420)

  • seizures(MP:0002064)

  • tonic-clonic seizures(MP:0003997)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse Pten genomic DNA
    Research applicationCancer Research
    Cell Biology Research
    Cre/loxP system
    General Purpose
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER (Tak W. Mak, Division of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute E-mail: tmak@uhnresearch.ca).
    DepositorShigeo Koyasu (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Renault-Mihara F, Mukaino M, Shinozaki M, Kumamaru H, Kawase S, Baudoux M, Ishibashi T, Kawabata S, Nishiyama Y, Sugai K, Yasutake K, Okada S, Nakamura M, Okano H.
    Regulation of RhoA by STAT3 coordinates glial scar formation.
    J Cell Biol 216(8) 2533-2550(2017) 28642362
    Kanayama M, Nakao K, Horie S, Aiba A.
    Generation of transgenic mouse line with prostate-specific expression of codon-improved Cre recombinase.
    Prostate Int 6(3) 99-103(2018) 30140659