Strain Data Sheet

RBRC02298

Strain Information

Image
BRC No.RBRC02298
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Il3<tm1Glli>
Former Common nameBALB/c-IL-3ノックアウトマウス
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainBALB/cAnNTac
Appearancealbino [A/A b/b c/c]
Strain development開発者／機関：Dr. Glenn Dranoff, Dana-Farber Cancer Institute, Harvard Medical school開発年：1998年入手経路：2005年2月にN9動物を入手D3 ES細胞を用いて作製されたノックアウト（129 x B6）をBALB/cに9代戻し交配をした状態で入手。その後BALB/cNTacに3代の戻し交配を行い、兄妹交配でホモ化した。
Strain descriptionIL-3は造血前駆細胞の肥満細胞、好塩基球の増殖因子である。ホモ欠損であっても交配の特性はBALB/cと同じである。接触皮膚炎や寄生虫感染時の肥満細胞の動因異常が報告されている。
Colony maintenance兄妹交配
ReferencesBlood, 91, 778-783 (1998). 9446636

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Il3Interleukin 311Il3<tm1Glli>targeted mutation 1, Stephen J GalliBPA, Csfmu, HCGF, Il-3, MCGF, PSF

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly Amouse PGK polyA11poly A

Ordering Information

供与核酸mouse Il3 genomic DNA, mouse Phosphoglycerate kinase-1(PGK) promoter, E. coli neo, mouse PGK polyA
Research applicationImmunology and Inflammation Research
提供条件条件を付加する。
利用者は事前に開発者の許可を得る。
開発者連絡先：Glenn Dranoff, MD, Harvard Medical School, Dana-Farber Cancer Institute E-mail: glenn_dranoff@dfci.harvard.edu.
Depositor小安　重夫（慶応義塾大学）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Bénard A, Mittelstädt A, Klösch B, Glanz K, Müller J, Schoen J, Nüse B, Brunner M, Naschberger E, Stürzl M, Mattner J, Muñoz LE, Sohn K, Grützmann R, Weber GF.
IL-3 orchestrates ulcerative colitis pathogenesis by controlling the development and the recruitment of splenic reservoir neutrophils.
Cell Rep 42(6) 112637(2023) 37300834

Motomura Y, Morita H, Moro K, Nakae S, Artis D, Endo TA, Kuroki Y, Ohara O, Koyasu S, Kubo M.
Basophil-derived interleukin-4 controls the function of natural helper cells, a member of ILC2s, in lung inflammation.
Immunity 40(5) 758-71(2014) 24837103

Balam S, Schiechl-Brachner G, Buchtler S, Halbritter D, Schmidbauer K, Talke Y, Neumayer S, Salewski JN, Winter F, Karasuyama H, Yamanishi Y, Renner K, Geissler EK, Mack M.
IL-3 Triggers Chronic Rejection of Cardiac Allografts by Activation of Infiltrating Basophils.
J Immunol 202(12) 3514-3523(2019) 31068389

Leyva-Castillo JM, Hener P, Michea P, Karasuyama H, Chan S, Soumelis V, Li M.
Skin thymic stromal lymphopoietin initiates Th2 responses through an orchestrated immune cascade.
Nat Commun 4 2847(2013) 24284909

Weber GF, Chousterman BG, He S, Fenn AM, Nairz M, Anzai A, Brenner T, Uhle F, Iwamoto Y, Robbins CS, Noiret L, Maier SL, Zönnchen T, Rahbari NN, Schölch S, Klotzsche-von Ameln A, Chavakis T, Weitz J, Hofer S, Weigand MA, Nahrendorf M, Weissleder R, Swirski FK.
Interleukin-3 amplifies acute inflammation and is a potential therapeutic target in sepsis.
Science 347(6227) 1260-5(2015) 25766237

Julia Ko¨lle, Theodor Zimmermann, Alexander Kiefer, Ralf J. Rieker, Paraskevi Xepapadaki, Sebastian Zundler, Nikolaos G. Papadopoulos, Susetta Finotto
Targeted deletion of Interleukin-3 results in asthma exacerbations
iScience 104440(2022) 35707726