Strain Data Sheet

RBRC02295

Strain Information

Image
BRC No.RBRC02295
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-p130Cas<tm1.2Homy> Tg(BLG-cre)1Homy
Former Common nameCas-ex2<flox/flox>-Tg(BLG-Cre)
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain development広島大学原爆放射線医科学研究所・本田浩章先生(2003)。
Strain descriptionCasp130 (Bcar1) 遺伝子のエクソン2 (SH3 domainをコード) をloxPで挟んだfloxアレルと乳腺特異的BLG (βlactoglobulin) -Creをもつダブルミュータントマウス。通常の維持繁殖には特に問題ない。ホモKOマウス、Tgマウスともに生存性、繁殖性あり。繁殖成績はあまり良くない。
Colony maintenanceKO: Homozygote x Homozygote; Tg: Carrier x Carrier [or Crossing to C57BL/6NCrlCrlj]
References
p130Cas, Crk-associated substrate plays essential roles in liver development by regulating sinusoidal endothelial cell fenestration.
Tazaki T, Sasaki T, Uto K, Yamasaki N, Tashiro S, Sakai R, Tanaka M, Oda H, Honda Z, Honda H
Hepatology, 52(3), 1089-1099 (2010). 20623582

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bcar1
MGI:108091
breast cancer anti-estrogen resistance 18Bcar1<tm1.2Homy> targeted mutation 1.2, Homy
Frt yeast FRT (flippase recombination target) site8Frt
loxP phage P1 loxP8loxP
loxP phage P1 loxP8loxP
cre Phage P1 Cre recombinaseUNcre sheep BLG promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
  • Detailed phenotype data

    Ordering Information

    供与核酸phage P1 loxP sites, yeast (Saccharomyces cerevisiae) FRT site, mouse Cas genomic DNA, sheep BLG genomic DNA, phage P1 Cre-recombinase
    Research applicationCell Biology Research
    Cre/loxP system
    FLP/frt system
    提供条件利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。
    Depositor本田 浩章(東京女子医科大学)
    Strain Status凍結精子のアイコン凍結精子
    Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data