Strain Information | |
---|---|
Image | ![]() |
BRC No. | RBRC02295 |
Type | Targeted Mutation and Transgene![]() |
Species | Mus musculus |
Strain name | B6.Cg-p130Cas<tm1.2Homy> Tg(BLG-cre)1Homy |
Former Common name | Cas-ex2<flox/flox>-Tg(BLG-Cre) |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | 広島大学原爆放射線医科学研究所・本田浩章先生(2003)。 |
Strain description | Casp130 (Bcar1) 遺伝子のエクソン2 (SH3 domainをコード) をloxPで挟んだfloxアレルと乳腺特異的BLG (βlactoglobulin) -Creをもつダブルミュータントマウス。通常の維持繁殖には特に問題ない。ホモKOマウス、Tgマウスともに生存性、繁殖性あり。繁殖成績はあまり良くない。 |
Colony maintenance | KO: Homozygote x Homozygote; Tg: Carrier x Carrier [or Crossing to C57BL/6NCrlCrlj] |
References | p130Cas, Crk-associated substrate plays essential roles in liver development by regulating sinusoidal endothelial cell fenestration. Tazaki T, Sasaki T, Uto K, Yamasaki N, Tashiro S, Sakai R, Tanaka M, Oda H, Honda Z, Honda H Hepatology, 52(3), 1089-1099 (2010). 20623582 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |||||||
---|---|---|---|---|---|---|---|
Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Bcar1 MGI:108091 | breast cancer anti-estrogen resistance 1 | 8 | Bcar1<tm1.2Homy> | targeted mutation 1.2, Homy | |||
Frt | yeast FRT (flippase recombination target) site | 8 | Frt | ||||
loxP | phage P1 loxP | 8 | loxP | ||||
loxP | phage P1 loxP | 8 | loxP | ||||
cre | Phage P1 Cre recombinase | UN | cre | sheep BLG promoter |
Phenotype | |
---|---|
Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
---|---|
供与核酸 | phage P1 loxP sites, yeast (Saccharomyces cerevisiae) FRT site, mouse Cas genomic DNA, sheep BLG genomic DNA, phage P1 Cre-recombinase |
Research application | Cell Biology Research Cre/loxP system FLP/frt system |
提供条件 | 利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。 |
Depositor | 本田 浩章(東京女子医科大学) |
Strain Status | ![]() |
Strain Availability | 凍結精子より作出したマウスを2~4ヶ月以内に提供可能 凍結精子を1ヶ月以内に提供可能 |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |