Strain Data Sheet

RBRC02295

Strain Information

Image
BRC No.RBRC02295
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-p130Cas<tm1.2Homy> Tg(BLG-cre)1Homy
Former Common nameCas-ex2<flox/flox>-Tg(BLG-Cre)
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain development広島大学原爆放射線医科学研究所・本田浩章先生（2003）。
Strain descriptionCasp130 (Bcar1) 遺伝子のエクソン2 (SH3 domainをコード) をloxPで挟んだfloxアレルと乳腺特異的BLG (βlactoglobulin) -Creをもつダブルミュータントマウス。通常の維持繁殖には特に問題ない。ホモKOマウス、Tgマウスともに生存性、繁殖性あり。繁殖成績はあまり良くない。
Colony maintenanceKO: Homozygote x Homozygote; Tg: Carrier x Carrier [or Crossing to C57BL/6NCrlCrlj]
ReferencesHepatology, 52(3), 1089-1099 (2010). 20623582

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bcar1breast cancer anti-estrogen resistance 18Bcar1<tm1.2Homy>targeted mutation 1.2, HomyCas, Crkas, p130 Cas

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcresheep BLG promoter

Ordering Information

供与核酸phage P1 loxP sites, yeast (Saccharomyces cerevisiae) FRT site, mouse Cas genomic DNA, sheep BLG genomic DNA, phage P1 Cre-recombinase
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
提供条件条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。
Depositor本田　浩章（東京女子医科大学）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data