Strain Data Sheet

RBRC02294

Strain Information

Image
BRC No.RBRC02294
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-p130Cas<tm1.2Homy>
Former Common nameCas-ex2<+/->
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Dr. Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2003.
Strain descriptionCasp130 (Crk-associated substrate p130) knockout mice. This mice were generated by the micro-injection of the vector to express Cre recombinase into the eggs of conditional KO strain that have flanked exon 2 encoding SH3 domain of Casp130. Homozygous mutant mice are embryonic lethal.
Colony maintenancebackcross to C57BL/6N (Heterozygote x C57BL/6NCrlCrlj)
References
p130Cas, Crk-associated substrate plays essential roles in liver development by regulating sinusoidal endothelial cell fenestration.
Tazaki T, Sasaki T, Uto K, Yamasaki N, Tashiro S, Sakai R, Tanaka M, Oda H, Honda Z, Honda H
Hepatology, 52(3), 1089-1099 (2010). 20623582

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bcar1breast cancer anti-estrogen resistance 18Bcar1targeted mutation 1.2, Homy
Frtyeast FRT (flippase recombination target) site8Frt
loxPphage P1 loxP8loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
  • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP site, yeast (Saccharomyces cerevisiae) FRT site, mouse Cas genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHiroaki Honda (Tokyo Women's Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)

    BRC mice in Publications

    No Data