Strain Data Sheet

RBRC02293

Strain Information

Image
BRC No.RBRC02293
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-p130Cas<tm1.1Homy>
Former Common nameCas-ex2<flox/flox>
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain development開発者／機関：本田浩章先生／広島大学原爆放射線医科学研究所開発年：2003年
Strain descriptionCas<p130> (Crk-associated substrate p130) 遺伝子のexon2をloxPで挟んだfloxマウス (exon2はSH3 domainをcodeしている) 。
Colony maintenance戻し交配Background系統名：C57BL/6N（日本チャールズリバー）
References
Functional analysis of Src homology 3-encoding exon (exon 2) of p130Cas in primary fibroblasts derived from exon 2-specific knockout mice.
Tazaki T, Miyazaki K, Hiyama E, Nakamoto T, Sakai R, Yamasaki N, Honda Z, Noda M, Miyasaka N, Sueda T, Honda H
Genes Cells., 13(2):145-57 (2008). 18233957

p130Cas, Crk-associated substrate plays essential roles in liver development by regulating sinusoidal endothelial cell fenestration.
Tazaki T, Sasaki T, Uto K, Yamasaki N, Tashiro S, Sakai R, Tanaka M, Oda H, Honda Z, Honda H
Hepatology, 52(3), 1089-1099 (2010). 20623582

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bcar1
MGI:108091		breast cancer anti-estrogen resistance 18Bcar1<tm1.1Homy> targeted mutation 1.1, Homy
Frt yeast FRT (flippase recombination target) site8Frt
loxP phage P1 loxP8loxP
loxP phage P1 loxP8loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • muscle phenotype(MP:0005369)

  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    供与核酸phage P1 loxP sites, yeast (Saccharomyces cerevisiae) FRT site, mouse Cas genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    FLP/frt system
    提供条件利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。
    Depositor本田　浩章（東京女子医科大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data