Strain Data Sheet

RBRC02291

Strain Information

Image
BRC No.RBRC02291
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6Cr-Tg(tetO-spH)CYoko
Former Common nameTetO-synaptopHluorin
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Mineto Yokoi, Kyoto University Graduate School of Medicine in 2005.
Strain descriptionThis transgenic mice express the GFP in the neuron axon terminal under the control of the tetracycline operator sequence.
Colony maintenanceHemizygote x Wild-type (C57BL/6CrSlc)
References
Striatal medium spiny neurons terminate in a distinct region in the lateral hypothalamic area and do not directly innervate orexin/hypocretin- or melanin-concentrating hormone-containing neurons.
Hiromi Sano, Mineto Yokoi
J. Neurosci., 27, 6948-6955 (2007) 17596443

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HBB2 rabbit beta-globin intron/polyA signalUNHBB2
Vamp2
MGI:1313277		vesicle-associated membrane protein 2UNVamp2
MGI:2179745		E. coli tetO (tetracycline operator)
  • neurodevelopmental disorder with hypotonia and autistic features with or without hyperkinetic movements(MedGEN)
    • pHluorin pH-sensitive Green Fluorescent Protein (Aequorea victoria)UNpHluorin

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAE. coli tetO, CMV minimal promoter, mouse vesicular membrane protein Vamp2 cDNA, Jellyfish pH-sensitive mutants GFP cDNA (pHluorin cDNA(Miesenbck, G., De Angelis, D.A., Rothman, J. E. Nature, 394, 192-195, 1998).), rabbit beta-globin intron/polyA signal
    Research applicationFluorescent Proteins/lacZ System
    General Purpose
    Neurobiology Research
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 27, 6948-6955 (2007). The RECIPIENT may only use the BIOLOGICAL RESOURCE only for non-commercial academic research purpose.
    DepositorYokoi Mineto (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data