Strain Data Sheet


Strain Information

BRC No.RBRC02291
SpeciesMus musculus
Strain nameC57BL/6Cr-Tg(tetO-spH)CYoko
Former Common nameTetO-synaptopHluorin
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Mineto Yokoi, Kyoto University Graduate School of Medicine in 2005.
Strain descriptionThis transgenic mice express the GFP in the neuron axon terminal under the control of the tetracycline operator sequence.
Colony maintenanceHemizygote x Wild-type (C57BL/6CrSlc)
ReferencesJ. Neurosci., 27, 6948-6955 (2007) 17596443

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Vamp2vesicle-associated membrane protein 2UNVamp2Syb-2, Syb2, synaptobrevin IIE. coli tetO (tetracycline operator)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin intron/polyA signalUNHBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
pHluorinpH-sensitive Green Fluorescent Protein (Aequorea victoria)UNpHluorin

Ordering Information

Donor DNAE. coli tetO, CMV minimal promoter, mouse vesicular membrane protein Vamp2 cDNA, Jellyfish pH-sensitive mutants GFP cDNA (pHluorin cDNA(Miesenbck, G., De Angelis, D.A., Rothman, J. E. Nature, 394, 192-195, 1998).), rabbit beta-globin intron/polyA signal
Research applicationFluorescent Proteins/lacZ System
General Purpose
Neurobiology Research
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J.Neurosci., 27, 6948-6955 (2007).The RECIPIENT may only use the BIOLOGICAL RESOURCE only for non-commercial academic research purpose.
DepositorYokoi Mineto (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data