Strain Information | |
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Image | |
BRC No. | RBRC02290 |
Type | Transgene |
Species | Mus musculus |
Strain name | STOCK Tg(Nanog-GFP,Puro)1Yam |
Former Common name | |
H-2 Haplotype | |
ES Cell line | RF8 [129S4/SvJae] |
Background strain | |
Appearance | |
Strain development | Developed by Dr. Shinya Yamanaka, Institute for Frontier Medical Sciences, Kyoto University in 2003. The construct was injected into RF8 ES cells derived from 129S4/SvJae. 129, C57BL/6 and DBA/2 mixed background. |
Strain description | Nanog-GFP transgenic mice. Nanog gene is a good candidate marker for maintaining pluripotent differentiation, because of the loss of pluripotent epiblast in Nanog KO mice, the tendency of Nanog-null ES cells to differenciate spontaneously, and the capability of Nanog-overexpressed ES cells to maintain self-renewal independently of leukaemia inhibitory factor (LIF). This Nanog-GFP transgene was generated by insertion of GFP-IRES-puromycin resistance gene (Puror) cassette into the 5' untranslated region of a bacterial artificial chromosome (BAC) containing the mouse Nanog gene. ES cells with the Nanog-GFP transgene were positive for GFP, but became negative when differentiation was induced. Using this system, pluripotent stem cells competent for germline chimaeras can be induced from Nanog-GFP transgenic mouse fibroblasts by introduction of candidate genes for pluripotency. Tg homozygous and hemizygous mice were viable and fertile. Male-biased sex ratios was observed (199 females : 261 males)(details unknown). |
Colony maintenance | Carrier x Carrier (Homozygote x Homozygote) |
References | Generation of germline-competent induced pluripotent stem cells. Okita K, Ichisaka T, Yamanaka S Nature, 448, 313-317 (2007) 17554338 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Frt | yeast FRT (flippase recombination target) site | UN | Frt | ||||
GFP | Green Fluorescent Protein (Aequorea victoria) | UN | Nanog promoter | ||||
GH | Growth hormone polyA (Bovine) | UN | GH | ||||
IRES | internal ribosomal entry site (EMCV) | UN | |||||
puro | puromycin acyltransferase (Streptomyces alboniger) | UN | puro |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Jellyfish EGFP cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES), S. alboniger puromycin resistance gene, bovine growth hormone polyadenylation signal, yeast FRT (flipase recombination target) site, mouse genomic DNA from RP23-117GFPNF |
Research application | Cell Biology Research Developmental Biology Research FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | |
Depositor | Shinya Yamanaka (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Dec 2007 Mouse of the Month Oct 2012 |
BRC mice in Publications |
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Nagamatsu G, Kosaka T, Saito S, Takubo K, Akiyama H, Sudo T, Horimoto K, Oya M, Suda T. Tracing the conversion process from primordial germ cells to pluripotent stem cells in mice. Biol Reprod 86(6) 182(2012) 22423052 |
Shinagawa T, Takagi T, Tsukamoto D, Tomaru C, Huynh LM, Sivaraman P, Kumarevel T, Inoue K, Nakato R, Katou Y, Sado T, Takahashi S, Ogura A, Shirahige K, Ishii S. Histone variants enriched in oocytes enhance reprogramming to induced pluripotent stem cells. Cell Stem Cell 14(2) 217-27(2014) 24506885 |
Nukaya D, Minami K, Hoshikawa R, Yokoi N, Seino S. Preferential gene expression and epigenetic memory of induced pluripotent stem cells derived from mouse pancreas. Genes Cells 20(5) 367-81(2015) 25727848 |
Nagamatsu G, Kosaka T, Kawasumi M, Kinoshita T, Takubo K, Akiyama H, Sudo T, Kobayashi T, Oya M, Suda T. A germ cell-specific gene, Prmt5, works in somatic cell reprogramming. J Biol Chem 286(12) 10641-8(2011) 21270127 |
Jincho Y, Araki R, Hoki Y, Tamura C, Nakamura M, Ando S, Kasama Y, Abe M. Generation of genome integration-free induced pluripotent stem cells from fibroblasts of C57BL/6 mice without c-Myc transduction. J Biol Chem 285(34) 26384-9(2010) 20554535 |
Araki R, Suga T, Hoki Y, Imadome K, Sunayama M, Kamimura S, Fujita M, Abe M. iPS cell generation-associated point mutations include many C > T substitutions via different cytosine modification mechanisms. Nat Commun 15(1) 4946(2024) 38862540 |
Araki R, Hoki Y, Suga T, Obara C, Sunayama M, Imadome K, Fujita M, Kamimura S, Nakamura M, Wakayama S, Nagy A, Wakayama T, Abe M. Genetic aberrations in iPSCs are introduced by a transient G1/S cell cycle checkpoint deficiency. Nat Commun 11(1) 197(2020) 31924765 |
Koga M, Matsuda M, Kawamura T, Sogo T, Shigeno A, Nishida E, Ebisuya M. Foxd1 is a mediator and indicator of the cell reprogramming process. Nat Commun 5 3197(2014) 24496101 |
Okita K, Hong H, Takahashi K, Yamanaka S. Generation of mouse-induced pluripotent stem cells with plasmid vectors. Nat Protoc 5(3) 418-28(2010) 20203661 |
Cavallari C, Fonsato V, Herrera MB, Bruno S, Tetta C, Camussi G. Role of Lefty in the anti tumor activity of human adult liver stem cells. Oncogene 32(7) 819-26(2013) 22469982 |
Iseki H, Nakachi Y, Hishida T, Yamashita-Sugahara Y, Hirasaki M, Ueda A, Tanimoto Y, Iijima S, Sugiyama F, Yagami K, Takahashi S, Okuda A, Okazaki Y. Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34(2) 322-33(2016) 26523946 |
Araki R, Hoki Y, Uda M, Nakamura M, Jincho Y, Tamura C, Sunayama M, Ando S, Sugiura M, Yoshida MA, Kasama Y, Abe M. Crucial role of c-Myc in the generation of induced pluripotent stem cells. Stem Cells 29(9) 1362-70(2011) 21732496 |
Araki R, Jincho Y, Hoki Y, Nakamura M, Tamura C, Ando S, Kasama Y, Abe M. Conversion of ancestral fibroblasts to induced pluripotent stem cells. Stem Cells 28(2) 213-20(2010) 20020427 |
Swistowski A, Peng J, Liu Q, Mali P, Rao MS, Cheng L, Zeng X. Efficient generation of functional dopaminergic neurons from human induced pluripotent stem cells under defined conditions. Stem Cells 28(10) 1893-904(2010) 20715183 |
Borisova E, Nishimura K, An Y, Takami M, Li J, Song D, Matsuo-Takasaki M, Luijkx D, Aizawa S, Kuno A, Sugihara E, Sato TA, Yumoto F, Terada T, Hisatake K, Hayashi Y. Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25(1) 103525(2022) 35106457 |