Strain Data Sheet

RBRC02277

Strain Information

Image
BRC No.RBRC02277
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nuak1<tm1Sia>/SiaRbrc
Former Common nameOmphk(lacZ) (Acc. No. CDB0029K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
A new serine/threonine protein kinase, Omphk1, essential to ventral body wall formation.
Mariko Hirano, Hiroshi Kiyonari, Ai Inoue, Kenryo Furushima, Takuya Murata, Yoko Suda, Shinichi Aizawa
Dev. Dyn., 235, 2229-2237 (2006). 16715502

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site10Frt
Frt yeast FRT (flippase recombination target) site10Frt
HBB2 rabbit beta-globin polyA10HBB2
Nuak1
MGI:1925226		NUAK family, SNF1-like kinase, 110Nuak1<tm1Sia>
MGI:3770654		targeted mutation 1, Shinichi Aizawa
lacZ beta-galactosidase (E. coli)10
lox71 mutant lox sequence10lox71
loxP phage P1 loxP10loxP
neo neomycin resistance gene (E. coli)10 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • herniated abdominal wall (MP:0000757)

  • omphalocele (MP:0003052)

  • perinatal lethality, complete penetrance (MP:0011089)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 phage lox71 site, E. coli lacZ, rabbit beta-globin polyA, yeast frt sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neo, P1 phage loxP site, mouse Pgk1 polyA, mouse Nuak1 genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. CDB0029K and the BIOLOGICAL RESOURCE is requested (https://large.riken.jp/distribution/mutant-list.html). (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested, Developmental Dynamics 235, 2229-2237 (2006). (3) Please inform the DEVELOPER (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data