Strain Data Sheet

RBRC02277

Information1

Image
BRC No.RBRC02277
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nuak1<tm1Sia>/SiaRbrc
Former Common nameOmphk(lacZ) (Acc. No. CDB0029K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesDev. Dyn., 235, 2229-2237 (2006). 16715502

Health Report

Examination Date / Room / Rack2020/11/09Room:3-ARack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nuak1NUAK family, SNF1-like kinase, 110Nuak1<tm1Sia>targeted mutation 1, Shinichi AizawaB230104P22Rik

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site10Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site10Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA10HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)10

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox71mutant lox sequence10lox71

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)10neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAP1 phage lox71 site, E. coli lacZ, rabbit beta-globin polyA, yeast frt sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neo, P1 phage loxP site, mouse Pgk1 polyA, mouse Nuak1 genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and Conditions(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. CDB0029K and the BIOLOGICAL RESOURCE is requested (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html). (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested, Developmental Dynamics 235, 2229-2237 (2006). (3) Please inform the DEVELOPER (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data