Strain Data Sheet

RBRC02251

Strain Information

Image
BRC No.RBRC02251
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Nrp2<tm1Mkl>
Former Common nameneuropilin2 Knock out mouse
H-2 Haplotype
ES Cell lineCGR8 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain development大阪大学大学院医学系研究科・高島成二先生（1998）。CGR8 ES細胞由来。
Strain descriptionNrp2遺伝子のノックアウトマウス。ATGを含むエクソン1とその下流の一部がlacZ-neoカセットで置換されている。ホモマウスは血管、神経系の異常により胎生期もしくは生後すぐに死亡する。
Colony maintenanceHetero mice are healthy and fertile. No specific care is needed. Homo mouse is dead in uterus or soon after birth.
References
Targeting of both mouse neuropilin-1 and neuropilin-2 genes severely impairs developmental yolk sac and embryonic angiogenesis.
Seiji Takashima, Masafumi Kitakaze, Masanori Asakura, Hiroshi Asanuma, Shoji Sanada, Fumi Tashiro, Hitoshi Niwa, Jun-ichi Miyazaki Ji, Seiichi Hirota, Yukihiko Kitamura, Takashi Kitsukawa, Hajime Fujisawa, Michael Klagsbrun, Masatsugu Hori
Proc. Natl. Acad. Sci. USA, 99, 3657-3662 (2002). 11891274

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Nrp2
MGI:1100492		neuropilin 21Nrp2<tm1Mkl>
MGI:2183238		targeted mutation 1, Michael Klagsbrun
lacZ beta-galactosidase (E. coli)1
neo neomycin resistance gene (E. coli)1

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal dorsal aorta morphology (MP:0004787)

  • abnormal nervous system development (MP:0003861)

  • abnormal neuronal migration (MP:0006009)

  • abnormal vascular development (MP:0000259)

  • abnormal vitelline vasculature morphology (MP:0003229)
    • more 3 phenotypes
  • decreased embryo size (MP:0001698)

  • embryonic lethality during organogenesis, complete penetrance (MP:0011098)

  • hemorrhage (MP:0001914)
    • Detailed phenotype data

    Ordering Information

    供与核酸E. coli lacZ, E. coli neo, Mouse Neuropilin2 genomic DNA
    Research applicationDevelopmental Biology Research
    Fluorescent Proteins/lacZ System
    Hematological Research
    Neurobiology Research
    提供条件利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。Proc. Natl. Acad. Sci. USA, 99, 3657-3662 (2002). 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
    Depositor高島　成二（国立大学法人大阪大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data