Strain Data Sheet

RBRC02240

Strain Information

Image
BRC No.RBRC02240
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Grid2<tm1Mim> Tg(Pcp2-Grid2*R514K)1Yuza
Former Common nameGluRδ2 rescue line-R/K
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentIn 2001, transgenic mice (Tg(PCP2-Grid2R/K)) lines were established by injection into fertilized FVB/N mouse eggs by the donating investigator at St. Jude Children’s Hospital.In 2002, the transgenic mice were bred on GluRδ2-/- background by crossing with B6-Grid2tm3Mish lines (RIKEN BRC No.01657).
Strain descriptionThe transgene (Grid2R/K), which has a mutation in the putative ligand binding site of the δ2 glutamate receptor, is expressed specifically in cerebellar Purkinje cells by a PCP2 promoter.
Colony maintenance繁殖効率：AThe mice are supplied as homozygotes for the transgene (Tg(PCP2-Grid2R/K)) on GluRδ2-/- background. Therefore, when crossed within a colony, no genotypings are necessary.
References
Rescue of abnormal phenotypes of the delta2 glutamate receptor-null mice by mutant delta2 transgenes.
Hirokazu Hirai, Taisuke Miyazaki, Wataru Kakegawa, Shinji Matsuda, Masayoshi Mishina, Masahiko Watanabe, Michisuke Yuzaki
EMBO reports, 6, 90-95 (2005). 15592450

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Grid2
MGI:95813		glutamate receptor, ionotropic, delta 26Grid2<tm1Mim>
MGI:2178052		targeted mutation 1, Masayoshi Mishina
  • autosomal recessive spinocerebellar ataxia 18(DisGeNET, MedGEN)
    • neo neomycin resistance gene (E. coli)6
    Grid2*R514K glutamate receptor, ionotropic, delta 2 (R514K mutant) cDNAUNGrid2*R514K Pcp2 promoter

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal CNS synapse formation (MP:0004996)

  • abnormal CNS synaptic transmission (MP:0002206)

  • abnormal GABA-mediated receptor currents (MP:0004008)

  • abnormal Purkinje cell innervation (MP:0003894)

  • abnormal Purkinje cell morphology (MP:0000877)
    • more 23 phenotypes
  • abnormal action potential (MP:0005402)

  • abnormal cerebellar molecular layer (MP:0000889)

  • abnormal cerebellum morphology (MP:0000849)

  • abnormal excitatory postsynaptic currents (MP:0002910)

  • abnormal eye blink conditioning behavior (MP:0002807)

  • abnormal eye movement (MP:0001389)

  • abnormal gait (MP:0001406)

  • abnormal locomotor activation (MP:0003313)

  • abnormal motor coordination/balance (MP:0001516)

  • abnormal optokinetic reflex (MP:0009619)

  • abnormal reflex (MP:0001961)

  • abnormal response to injury (MP:0005164)

  • abnormal vestibuloocular reflex (MP:0004844)

  • absent long-term depression (MP:0001899)

  • ataxia (MP:0001393)

  • decreased locomotor activity (MP:0001402)

  • decreased vertical activity (MP:0002757)

  • head tilt (MP:0005191)

  • impaired coordination (MP:0001405)

  • impaired righting response (MP:0001523)

  • increased miniature inhibitory postsynaptic current amplitude (MP:0014384)

  • increased prepulse inhibition (MP:0009141)

  • thin cerebellar molecular layer (MP:0000890)
    • Detailed phenotype data

    Ordering Information

    供与核酸mouse PCP2 promoter, mouse Grid2 cDNA
    Research applicationNeurobiology Research
    提供条件利用者は事前に寄託者の提供承諾書を得る。GluRd2KOマウスの使用に際しては、GluRδ2KO mouse（BRC No.01657）の下記の寄託条件の範囲内で利用する。「寄託者の提供承諾書を得る。研究成果の公表にあたって謝辞の表明を必要とする。利用者が本リソースを用いて得た成果に基づき特許、知的所有権、または他の権利を申請する場合には、寄託者の同意を必要とする。本リソースの使用は共同研究のみに限定する。寄託後2年以内の論文発表あるいは寄託後2年以内に論文発表が無い場合はその後の論文発表の際には、利用者は寄託者を共著者とする必要がある。」
    Depositor柚﨑　通介（慶應義塾大学）
    Strain Status凍結胚のアイコン凍結胚
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data