Strain Data Sheet

RBRC02240

Information1

Image
BRC No.RBRC02240
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Grid2<tm1Mim> Tg(Pcp2-Grid2*R514K)1Yuza
Former Common nameGluRδ2 rescue line-R/K
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentIn 2001, transgenic mice (Tg(PCP2-Grid2R/K)) lines were established by injection into fertilized FVB/N mouse eggs by the donating investigator at St. Jude Children's Hospital.In 2002, the transgenic mice were bred on GluR 2-/- background by crossing with B6-Grid2<tm3Mish> lines (RIKEN BRC No.01657).
Strain descriptionThe transgene (Grid2R/K), which has a mutation in the putative ligand binding site of the δ2 glutamate receptor, is expressed specifically in cerebellar Purkinje cells by a PCP2 promoter.
Colony maintenanceThe mice are supplied as homozygotes for the transgene (Tg(PCP2-Grid2R/K)) on GluRδ2-/- background. Therefore, when crossed within a colony, no genotypings are necessary.
ReferencesEMBO reports, 6, 90-95 (2005). 15592450

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Grid2glutamate receptor, ionotropic, delta 26Grid2<tm1Mim>targeted mutation 1, Masayoshi MishinaB230104L07Rik, GluRdelta2, tpr

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Grid2*R514Kglutamate receptor, ionotropic, delta 2 (R514K mutant) cDNAUNGrid2*R514KPcp2 promoter

Information2

Donor DNAmouse PCP2 promoter, mouse Grid2 cDNA
Research applicationNeurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the GluR 2KO mouse (BRC No. 01657) complying with the following terms and conditions requested by the DEPOSITOR of the GluR 2KO mouse "The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. And Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for first publication if no publication is made within the TWO years."
DepositorMichisuke Yuzaki (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data