Strain Data Sheet

RBRC02231

Strain Information

Image
BRC No.RBRC02231
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Atg5<tm1Nmz>
Former Common name
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Masahiko Hatano and Takeshi Tokuhisa, Graduate School of Medicine, Chiba University, and Noboru Mizushima, National Institute for Basic Biology in 2003. The knockout construct was electoroporated into R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+>. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionAtg5 knockout mice. Exons 1 and 2 of Atg5 were replaced with a neomycin resistance gene. Homozygous mutant mice die during the early neonatal period due to a lack of nutrients. Autophagy is an intracellular degradation process by an autophagosome, which contains a portion of cytoplasm and subsequently degrades upon fusion with a lysosome. Autophagy is considered to be important for the cellular response to starvation and the normal turnover of cytoplasmic components as well. Atg5, autophagy-related 5 is essential for autophagosome formation. Conditional mice (floxed allele) are also available: B6.129S-Atg5<tm1Myok> (RBRC02975).
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
References
The role of autophagy during the early neonatal starvation period.
Kuma A, Hatano M, Matsui M, Yamamoto A, Nakaya H, Yoshimori T, Ohsumi Y, Tokuhisa T, Mizushima N
Nature, 432, 1032-1036 (2004). 15525940

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Atg5autophagy related 510Atg5targeted mutation 1, Noboru Mizushima
  • spinocerebellar ataxia, autosomal recessive 25(MedGEN)
  • neoneomycin resistance gene (E. coli)10herpes simplex virus thymidine kinase promoter (HSV tk promoter)
  • spinocerebellar ataxia, autosomal recessive 25(MedGEN)
  • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal ST segment(MP:0003897)

  • abnormal T cell morphology(MP:0008037)

  • abnormal T cell physiology(MP:0002444)

  • abnormal T cell subpopulation ratio(MP:0003944)

  • abnormal adenohypophysis morphology(MP:0004163)
  • more 25 phenotypes
  • abnormal adrenal gland morphology(MP:0000639)

  • abnormal amino acid level(MP:0005332)

  • abnormal autophagy(MP:0008260)

  • abnormal brain morphology(MP:0002152)

  • abnormal circulating amino acid level(MP:0005311)

  • abnormal hepatocyte morphology(MP:0000607)

  • abnormal liver morphology(MP:0000598)

  • abnormal lysosome morphology(MP:0005058)

  • abnormal thymus cortex morphology(MP:0002371)

  • abnormal thymus morphology(MP:0000703)

  • abnormal thymus physiology(MP:0003763)

  • abnormal utricle morphology(MP:0006090)

  • absent gastric milk in neonates(MP:0009546)

  • absent otoliths(MP:0002978)

  • decreased CD4-positive, alpha-beta T cell number(MP:0008075)

  • decreased CD8-positive, alpha-beta T cell number(MP:0008079)

  • decreased T cell number(MP:0005018)

  • decreased T cell proliferation(MP:0005095)

  • enlarged otoliths(MP:0003143)

  • hematopoietic system phenotype(MP:0005397)

  • increased mitochondrial size(MP:0011630)

  • neonatal lethality, complete penetrance(MP:0011087)

  • neuronal intranuclear inclusions(MP:0004191)

  • oxidative stress(MP:0003674)

  • vision/eye phenotype(MP:0005391)
  • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Atg5 genomic DNA
    Research applicationCell Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 432, 1032-1036 (2004). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorNoboru Mizushima (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-
    Mouse of the Month Jul 2009

    BRC mice in Publications

    Mizushima N, Yoshimori T, Levine B.
    Methods in mammalian autophagy research.
    Cell 140(3) 313-26(2010) 20144757
    Najafov A, Luu HS, Mookhtiar AK, Mifflin L, Xia HG, Amin PP, Ordureau A, Wang H, Yuan J.
    RIPK1 Promotes Energy Sensing by the mTORC1 Pathway.
    Mol Cell 81(2) 370-385.e7(2021) 33271062
    Wu Y, Li Y, Zhang H, Huang Y, Zhao P, Tang Y, Qiu X, Ying Y, Li W, Ni S, Zhang M, Liu L, Xu Y, Zhuang Q, Luo Z, Benda C, Song H, Liu B, Lai L, Liu X, Tse HF, Bao X, Chan WY, Esteban MA, Qin B, Pei D.
    Autophagy and mTORC1 regulate the stochastic phase of somatic cell reprogramming.
    Nat Cell Biol 17(6) 715-25(2015) 25985393