Strain Data Sheet

RBRC02220

Strain Information

Image
BRC No.RBRC02220
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Mafb<tm(GFP)UTR>
Former Common name
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
MafB is essential for renal development and F4/80 expression in macrophages.
Takashi Moriguchi, Michito Hamada, Naoki Morito, Tsumoru Terunuma, Kazuteru Hasegawa, Chuan Zhang, Tomomasa Yokomizo, Ritsuko Esaki, Etsushi Kuroda, Keigyou Yoh, Takashi Kudo, Michio Nagata, David R Greaves, James Douglas Engel, Masayuki Yamamoto, Satoru Takahashi
Mol. Cell Biol., 26, 5715-5727 (2006). 16847325

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)2
Mafb
MGI:104555		MAF bZIP transcription factor B2Mafb<tm(GFP)UTR> targeted mutation
  • Duane retraction syndrome 3 with or without deafness(MedGEN)

  • multicentric carpo-tarsal osteolysis with or without nephropathy(MedGEN)
    • loxP phage P1 loxP2loxP
    loxP phage P1 loxP2loxP
    neo neomycin resistance gene (E. coli)2 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal kidney development (MP:0000527)

  • abnormal podocyte foot process morphology (MP:0008059)

  • abnormal renal/urinary system morphology (MP:0000516)

  • abnormal seminiferous tubule epithelium morphology (MP:0011750)

  • absent urinary bladder (MP:0009252)
    • more 9 phenotypes
  • decreased macrophage cell number (MP:0003884)

  • increased circulating creatinine level (MP:0005553)

  • increased kidney apoptosis (MP:0011368)

  • increased podocyte apoptosis (MP:0011282)

  • increased renal glomerulus apoptosis (MP:0011369)

  • kidney atrophy (MP:0004970)

  • kidney hemorrhage (MP:0000533)

  • renal glomerulus cyst (MP:0011682)

  • renal tubular necrosis (MP:0004154)
    • Detailed phenotype data

    Ordering Information

    Donor DNAjellyfish GFP cDNA, P1 phage loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Pgk poly A additional signal, mouse MafB gene
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    General Purpose
    Sensorineural Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 26, 5715-5727 (2006).
    DepositorSatoru Takahashi (University of Tsukuba)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data