Strain Data Sheet

RBRC02220

Information1

Image
BRC No.RBRC02220
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Mafb<tm(GFP)UTR>
Former Common name
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesMol. Cell Biol., 26, 5715-5727 (2006). 16847325

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mafbv-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian)2Mafb<tm(GFP)UTR>targeted mutationKreisler, Krml, Krml1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2neo; neomycin;

Information2

Donor DNAMouse MafB gene, Mouse PGK promoter, E. coli neo, PGK poly A additional signal、P1 phage loxP sequence, jellyfish GFP cDNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
General Purpose
Sensorineural Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. cell. Biol., 26, 5715-5727 (2006).
DepositorSatoru Takahashi (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP

BRC mice in Publications

No Data