Strain Data Sheet

RBRC02192

update : 2024-04-19

Strain Information
Image
BRC No.	RBRC02192
Type	Targeted Mutation
Species	Mus musculus
Strain name	B6.129-Mapk14<tm1.2Otsu>
Former Common name	p38α-flox マウス
H-2 Haplotype
ES Cell line	R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain	C57BL/6JJcl
Appearance	black [a/a B/B C/C]
Strain development	p38αコンベンショナルノックアウトマウスが胎生致死であるため、生体でのp38αの機能を評価する目的で開発した。開発年：2000年　開発者：大津欣也先生　機関名：大阪大学大学院医学系研究科
Strain description	p38αfloxマウスは、p38αfloxATP結合部位を含むエクソンの両端イントロン部分にloxP配列を導入。Creリコンビナーゼ発現細胞でp38αをノックアウトできる。
Colony maintenance	C57BL6背景のホモp38αfloxマウス同士の交配で系統を維持している。
References	Mol. Cell. Biol., 24, 10611-10620 (2004). 15572667

Health Report
Examination Date / Room / Rack

Gene
Gene info	Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Mapk14mitogen activated protein kinase 1417Mapk14<tm1.2Otsu>targeted mutation 1.2, Kinya OtsuCrk1, Csbp1, CSBP2, Mxi2, p38, p38 MAP Kinase, p38-alpha, p38MAPK, tRNA synthetase cofactor p38 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP

Gene

Gene info

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

Mapk14mitogen activated protein kinase 1417Mapk14<tm1.2Otsu>targeted mutation 1.2, Kinya OtsuCrk1, Csbp1, CSBP2, Mxi2, p38, p38 MAP Kinase, p38-alpha, p38MAPK, tRNA synthetase cofactor p38

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

loxPphage P1 loxP17loxP

Ordering Information
供与核酸	Phage P1 loxP sites, mouse p38 alpha genomic DNA
Research application	Cell Biology Research Cre/loxP system General Purpose
提供条件	条件を付加する。利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。Mol. Cell. Biol., 24, 10611-10620 (2004). 研究成果の公表にあたって謝辞の表明を必要とする。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請及び事業活動を行う場合は、寄託者と別途協議を行う。
Depositor	大津　欣也（国立大学法人大阪大学）
Strain Status	凍結胚凍結精子
Strain Availability	凍結胚より作出したマウスを2~4ヶ月以内に提供可能凍結精子を1ヶ月以内に提供可能凍結胚を1ヶ月以内に提供可能
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Lab HP (Japanese) Genotyping protocol -PCR- Genetic Background Mouse of the Month Jul 2007

BRC mice in Publications

Luo Q, Schnöder L, Hao W, Litzenburger K, Decker Y, Tomic I, Menger MD, Liu Y, Fassbender K. p38α-MAPK-deficient myeloid cells ameliorate symptoms and pathology of APP-transgenic Alzheimer's disease mice. Aging Cell 21(8) e13679(2022) 35909315 Krementsov DN, Noubade R, Dragon JA, Otsu K, Rincon M, Teuscher C. Sex-specific control of central nervous system autoimmunity by p38 mitogen-activated protein kinase signaling in myeloid cells. Ann Neurol 75(1) 50-66(2014) 24027119 Schnöder L, Tomic I, Schwindt L, Helm D, Rettel M, Schulz-Schaeffer W, Krause E, Rettig J, Fassbender K, Liu Y. P38α-MAPK phosphorylates Snapin and reduces Snapin-mediated BACE1 transportation in APP-transgenic mice. FASEB J 35(7) e21691(2021) 34118085 Schnöder L, Gasparoni G, Nordström K, Schottek A, Tomic I, Christmann A, Schäfer KH, Menger MD, Walter J, Fassbender K, Liu Y. Neuronal deficiency of p38α-MAPK ameliorates symptoms and pathology of APP or Tau-transgenic Alzheimer's mouse models. FASEB J 34(7) 9628-9649(2020) 32475008 Schnöder L, Hao W, Qin Y, Liu S, Tomic I, Liu X, Fassbender K, Liu Y. Deficiency of Neuronal p38α MAPK Attenuates Amyloid Pathology in Alzheimer Disease Mouse and Cell Models through Facilitating Lysosomal Degradation of BACE1. J Biol Chem 291(5) 2067-79(2016) 26663083 Liu X, Ma B, Malik AB, Tang H, Yang T, Sun B, Wang G, Minshall RD, Li Y, Zhao Y, Ye RD, Xu J. Bidirectional regulation of neutrophil migration by mitogen-activated protein kinases. Nat Immunol 13(5) 457-64(2012) 22447027 Hutchison MR. Mice with a conditional deletion of the neurotrophin receptor TrkB are dwarfed, and are similar to mice with a MAPK14 deletion. PLoS One 8(6) e66206(2013) 23776632 Kase Y, Otsu K, Shimazaki T, Okano H. Involvement of p38 in Age-Related Decline in Adult Neurogenesis via Modulation of Wnt Signaling. Stem Cell Reports 12(6) 1313-1328(2019) 31080114

BRC mice in Publications

Luo Q, Schnöder L, Hao W, Litzenburger K, Decker Y, Tomic I, Menger MD, Liu Y, Fassbender K.
p38α-MAPK-deficient myeloid cells ameliorate symptoms and pathology of APP-transgenic Alzheimer's disease mice.
Aging Cell 21(8) e13679(2022) 35909315

Krementsov DN, Noubade R, Dragon JA, Otsu K, Rincon M, Teuscher C.
Sex-specific control of central nervous system autoimmunity by p38 mitogen-activated protein kinase signaling in myeloid cells.
Ann Neurol 75(1) 50-66(2014) 24027119

Schnöder L, Tomic I, Schwindt L, Helm D, Rettel M, Schulz-Schaeffer W, Krause E, Rettig J, Fassbender K, Liu Y.
P38α-MAPK phosphorylates Snapin and reduces Snapin-mediated BACE1 transportation in APP-transgenic mice.
FASEB J 35(7) e21691(2021) 34118085

Schnöder L, Gasparoni G, Nordström K, Schottek A, Tomic I, Christmann A, Schäfer KH, Menger MD, Walter J, Fassbender K, Liu Y.
Neuronal deficiency of p38α-MAPK ameliorates symptoms and pathology of APP or Tau-transgenic Alzheimer's mouse models.
FASEB J 34(7) 9628-9649(2020) 32475008

Schnöder L, Hao W, Qin Y, Liu S, Tomic I, Liu X, Fassbender K, Liu Y.
Deficiency of Neuronal p38α MAPK Attenuates Amyloid Pathology in Alzheimer Disease Mouse and Cell Models through Facilitating Lysosomal Degradation of BACE1.
J Biol Chem 291(5) 2067-79(2016) 26663083

Liu X, Ma B, Malik AB, Tang H, Yang T, Sun B, Wang G, Minshall RD, Li Y, Zhao Y, Ye RD, Xu J.
Bidirectional regulation of neutrophil migration by mitogen-activated protein kinases.
Nat Immunol 13(5) 457-64(2012) 22447027

Hutchison MR.
Mice with a conditional deletion of the neurotrophin receptor TrkB are dwarfed, and are similar to mice with a MAPK14 deletion.
PLoS One 8(6) e66206(2013) 23776632

Kase Y, Otsu K, Shimazaki T, Okano H.
Involvement of p38 in Age-Related Decline in Adult Neurogenesis via Modulation of Wnt Signaling.
Stem Cell Reports 12(6) 1313-1328(2019) 31080114

Strain Information

Health Report

Gene

Ordering Information

BRC mice in Publications