Strain Data Sheet
RBRC02189
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC02189 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6Cr-Tg(Pcdh21-cre)BYoko |
| Former Common name | Pcdh21-nCre, C57BL/6 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6NCrSlc |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Dr. Mineto Yokoi Kyoto University Guraduate School of Medicine nin 2004. |
| Strain description | This transgenic mice express the cre recombinase gene under the control of the Pcdh21 gene promoter. This can be used to make an conditional knock-out by crossing with the other genetically-engineered mice that have floxed genes. The expression of cre recombinase was observed in the mitral/tufted cells that are projection neurons in the olfactory bulb. |
| Colony maintenance | Backcross to C57BL/6 (Hemizygote x C57BL/6CrSlc) |
| References | Transgenic expression of Cre recombinase in mitral/tufted cells of the olfactory bulb. Yumiko Nagai, Hiromi Sano, Mineto Yokoi Genesis, 43, 12-16 (2005). 16106355 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| cre | Phage P1 Cre recombinase | UN | cre | Pcdh21 promoter | |||
| tTA | tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain) | UN | tTA | Pcdh21 promoter | |||
Phenotype | |
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| Phenotype annotation from literatures by Mammalian phenotype ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | FLAG tag, tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain), human FGF2-IRES, nuclear localization signal, Phage P1 Cre recombinase, rabbit beta-globin intron/polyA signal, mouse pcdh21 genomic DNA |
| Research application | Cre/loxP system Neurobiology Research Tet system Cre driver |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 43, 12-16 (2005). |
| Depositor | Yokoi Mineto (Kyoto University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Inagaki S, Nakagawa-Tamagawa N, Huynh NZ, Kambe Y, Yagasaki R, Manita S, Fujimoto S, Noda T, Mori M, Teranishi A, Takeshima H, Ishikawa K, Naitou Y, Yokoyama T, Sakamoto M, Hayashi K, Kitamura K, Tagawa Y, Okuda S, Sato TK, Imai T. Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo. Nat Methods 23(4) 839-853(2026) 41820664 |
Leiwe MN, Fujimoto S, Baba T, Moriyasu D, Saha B, Sakaguchi R, Inagaki S, Imai T. Automated neuronal reconstruction with super-multicolour Tetbow labelling and threshold-based clustering of colour hues. Nat Commun 15(1) 5279(2024) 38918382 |
Fujimoto S, Leiwe MN, Aihara S, Sakaguchi R, Muroyama Y, Kobayakawa R, Kobayakawa K, Saito T, Imai T. Activity-dependent local protection and lateral inhibition control synaptic competition in developing mitral cells in mice. Dev Cell (2023) 37290446 |
Sha MFR, Koga Y, Murata Y, Taniguchi M, Yamaguchi M. Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle. Front Neurosci 17 1247375(2023) 37680965 |
Inoue N, Nishizumi H, Ooyama R, Mogi K, Nishimori K, Kikusui T, Sakano H. The olfactory critical period is determined by activity-dependent Sema7A/PlxnC1 signaling within glomeruli. Elife 10 (2021) 33780330 |
Sato T, Homma R, Nagayama S. Direct Comparison of Odor Responses of Homologous Glomeruli in the Medial and Lateral Maps of the Mouse Olfactory Bulb. eNeuro 7(2) (2020) 31974110 |
Iwata R, Kiyonari H, Imai T. Mechanosensory-Based Phase Coding of Odor Identity in the Olfactory Bulb. Neuron 96(5) 1139-1152.e7(2017) 29216451 |
Miller JE, Granados-Fuentes D, Wang T, Marpegan L, Holy TE, Herzog ED. Vasoactive intestinal polypeptide mediates circadian rhythms in mammalian olfactory bulb and olfaction. J Neurosci 34(17) 6040-6(2014) 24760863 |