Strain Data Sheet

RBRC02189

Strain Information

Image
BRC No.RBRC02189
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6Cr-Tg(Pcdh21-cre)BYoko
Former Common namePcdh21-nCre, C57BL/6
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Mineto Yokoi Kyoto University Guraduate School of Medicine nin 2004.
Strain descriptionThis transgenic mice express the cre recombinase gene under the control of the Pcdh21 gene promoter. This can be used to make an conditional knock-out by crossing with the other genetically-engineered mice that have floxed genes. The expression of cre recombinase was observed in the mitral/tufted cells that are projection neurons in the olfactory bulb.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6CrSlc)
References
Transgenic expression of Cre recombinase in mitral/tufted cells of the olfactory bulb.
Yumiko Nagai, Hiromi Sano, Mineto Yokoi
Genesis, 43, 12-16 (2005). 16106355

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
cre Phage P1 Cre recombinaseUNcre Pcdh21 promoter
tTA tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTA Pcdh21 promoter

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • obsolete no phenotypic analysis (MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAFLAG tag, tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain), human FGF2-IRES, nuclear localization signal, Phage P1 Cre recombinase, rabbit beta-globin intron/polyA signal, mouse pcdh21 genomic DNA
    Research applicationCre/loxP system
    Neurobiology Research
    Tet system
    Cre driver
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 43, 12-16 (2005).
    DepositorYokoi Mineto (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Leiwe MN, Fujimoto S, Baba T, Moriyasu D, Saha B, Sakaguchi R, Inagaki S, Imai T.
    Automated neuronal reconstruction with super-multicolour Tetbow labelling and threshold-based clustering of colour hues.
    Nat Commun 15(1) 5279(2024) 38918382
    Fujimoto S, Leiwe MN, Aihara S, Sakaguchi R, Muroyama Y, Kobayakawa R, Kobayakawa K, Saito T, Imai T.
    Activity-dependent local protection and lateral inhibition control synaptic competition in developing mitral cells in mice.
    Dev Cell (2023) 37290446
    Sha MFR, Koga Y, Murata Y, Taniguchi M, Yamaguchi M.
    Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle.
    Front Neurosci 17 1247375(2023) 37680965
    Inoue N, Nishizumi H, Ooyama R, Mogi K, Nishimori K, Kikusui T, Sakano H.
    The olfactory critical period is determined by activity-dependent Sema7A/PlxnC1 signaling within glomeruli.
    Elife 10 (2021) 33780330
    Sato T, Homma R, Nagayama S.
    Direct Comparison of Odor Responses of Homologous Glomeruli in the Medial and Lateral Maps of the Mouse Olfactory Bulb.
    eNeuro 7(2) (2020) 31974110
    Iwata R, Kiyonari H, Imai T.
    Mechanosensory-Based Phase Coding of Odor Identity in the Olfactory Bulb.
    Neuron 96(5) 1139-1152.e7(2017) 29216451
    Miller JE, Granados-Fuentes D, Wang T, Marpegan L, Holy TE, Herzog ED.
    Vasoactive intestinal polypeptide mediates circadian rhythms in mammalian olfactory bulb and olfaction.
    J Neurosci 34(17) 6040-6(2014) 24760863