Strain Data Sheet

RBRC02188

Information1

Image
BRC No.RBRC02188
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6Cr-Tg(tetO-APTTC)1Yoko
Former Common nameB6.tetO-APTTC,C57BL/6
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Mineto Yokoi, Kyoto University Graduate School of Medicine in 2004. The transgene was injected into the pronuclei of C57BL/6CrSlc fertilized eggs.
Strain descriptionThis transgenic mice express a fusion gene, APTTC, consisting of the truncated human placental alkaline phosphatase with TTC (Tetanus toxin C fragment). This strain can be used to make an inducible expression of APTTC as a retrograde transynaptic genetic tracer corssing with the genetically-engineered mice that have a tetracycline-controlled transactivator (tTA) in any specific neurons.
Colony maintenanceCarrier x Noncarrier [C57BL/6CrSlc]Tg/+ hemizygous mice are viable and fertile.
ReferencesGenesis, 45, 123-128 (2007). 17299748

Health Report

Examination Date / Room / Rack2007/08/20Room:3-CRack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
PLAPhuman placental alkaline phosphataseUNPLAPE. coli tetO (tetracycline operator)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
TTCnon-toxic c fragment (Clostridium tetani)UNTTC

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signalUN

Information2

Donor DNAE. coli tetracycline operator(tetO) promoter, artificial intron, splice site, human placental alkaline phosphatase cDNA, Clostridium tetani non-toxic c fragment, SV40 polyA signal
Research applicationNeurobiology Research
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 45, 123-128 (2007).
DepositorYokoi Mineto (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data