Strain Data Sheet

RBRC02097

Strain Information

Image
BRC No.RBRC02097
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Izumo1<tm1Osb>
Former Common nameIzumo KO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Drs. Naokazu Inoue and Masaru Okabe at Osaka University in 2003. 129, C57BL/6 and DBA/2 mixed background.
Strain descriptionIzumo1 is a immunoglobulin super family protein that is required for sperm to fuse with eggs. Homozygous mutant mice are viable but males are sterile. The Izumo-deficient sperm can bind to an egg, and even burrow through the zona pellucida, but are incapable of fusing with egg.
Colony maintenanceSibling Mating (Hemizygote x Wild-type, Hemizygote) Homozygous mutant males are sterile.
References
The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs.
Inoue N, Ikawa M, Isotani A, Okabe M
Nature, 434, 234-238 (2005). 15759005

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Izumo1izumo sperm-egg fusion 17Izumo1targeted mutation 1, Research Institute for Microbial Diseases, Osaka University
loxPphage P1 loxP7loxP
loxPphage P1 loxP7loxP
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • impaired fertilization(MP:0000242)

  • male infertility(MP:0001925)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse PGK promoter, E. coli neo, mouse Izumo1 genomic DNA
    Research applicationCre/loxP system
    Reproductive Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 434, 234-238 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the BIOLOGICAL RESOURCES. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasaru Okabe (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data