Strain Data Sheet

RBRC02095

Strain Information

Image
BRC No.RBRC02095
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(Th-EGFP)21-31Koba
Former Common nameB6.Cg-Tg(TH-GFP)21-31
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain development福島県立医科大学医学部・小林和人先生(1998)。B6D2F2受精卵へインジェクションにより作出。C57BL/6Jに戻し交配された。2ラインあり。RBRC02095(メインライン)、RBRC03162。
Strain descriptionラットチロシン水酸化酵素 (TH) 遺伝子プロモーターに、オワンクラゲ緑色蛍光タンパク質 (GFP) 遺伝子を接続した導入遺伝子を持つ。Tgマウスは脳内および末梢器官のカテコールアミン合成細胞がGFPにより標識される。ホモマウスは致死 (原因は不明) 。
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesJ. Neurochem., 82, 295-304 (2002). 12124430

Health Report

Examination Date / Room / Rack2024/02/19Room:4-ARack:K
2023/11/20Room:4-ARack:K
2023/08/21Room:4-ARack:K
2023/05/22Room:4-ARack:K
2023/02/20Room:4-ARack:K
2022/11/21Room:4-ARack:K
2022/08/22Room:4-ARack:K
2022/05/23Room:4-ARack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNrat tyrosine hydroxylase (TH) promoter

Ordering Information

供与核酸rat tyrosine hydroxylase (TH) promoter, rabbit beta-globin intron, jelly fish green fluorescent protein (GFP) gene, rabbit beta-globin poly A, SV40 early gene poly A
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。J. Neurochem., 82, 295-304 (2002).
Depositor小林 和人(公立大学法人福島県立医科大学)
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
生体マウスを1~3ヶ月以内に提供可能
Additional Info.Construct map
Transgene sequence
Mouse of the Month May 2008
Genetic Background
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

Heo JY, Nam MH, Yoon HH, Kim J, Hwang YJ, Won W, Woo DH, Lee JA, Park HJ, Jo S, Lee MJ, Kim S, Shim JE, Jang DP, Kim KI, Huh SH, Jeong JY, Kowall NW, Lee J, Im H, Park JH, Jang BK, Park KD, Lee HJ, Shin H, Cho IJ, Hwang EM, Kim Y, Kim HY, Oh SJ, Lee SE, Paek SH, Yoon JH, Jin BK, Kweon GR, Shim I, Hwang O, Ryu H, Jeon SR, Lee CJ.
Aberrant Tonic Inhibition of Dopaminergic Neuronal Activity Causes Motor Symptoms in Animal Models of Parkinson's Disease.
Curr Biol 30(2) 276-291.e9(2020) 31928877

Masi A, Narducci R, Resta F, Carbone C, Kobayashi K, Mannaioni G.
Differential contribution of Ih to the integration of excitatory synaptic inputs in substantia nigra pars compacta and ventral tegmental area dopaminergic neurons.
Eur J Neurosci 42(9) 2699-706(2015) 26354486

Kiyokage E, Kobayashi K, Toida K.
Spatial distribution of synapses on tyrosine hydroxylase-expressing juxtaglomerular cells in the mouse olfactory glomerulus.
J Comp Neurol 525(5) 1059-1074(2017) 27864931

Suzuki Y, Kiyokage E, Sohn J, Hioki H, Toida K.
Structural basis for serotonergic regulation of neural circuits in the mouse olfactory bulb.
J Comp Neurol 523(2) 262-80(2015) 25234191

Sawada M, Kaneko N, Inada H, Wake H, Kato Y, Yanagawa Y, Kobayashi K, Nemoto T, Nabekura J, Sawamoto K.
Sensory input regulates spatial and subtype-specific patterns of neuronal turnover in the adult olfactory bulb.
J Neurosci 31(32) 11587-96(2011) 21832189

Zhu S, Zhao C, Wu Y, Yang Q, Shao A, Wang T, Wu J, Yin Y, Li Y, Hou J, Zhang X, Zhou G, Gu X, Wang X, Bustelo XR, Zhou J.
Identification of a Vav2-dependent mechanism for GDNF/Ret control of mesolimbic DAT trafficking.
Nat Neurosci 18(8) 1084-93(2015) 26147533

Kosaka T, Pignatelli A, Kosaka K.
Heterogeneity of tyrosine hydroxylase expressing neurons in the main olfactory bulb of the mouse.
Neurosci Res 157 15-33(2020) 31629793