Strain Information | |
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Image | |
BRC No. | RBRC02078 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129P2-Slp2<tm1Mitf> |
Former Common name | Slp2-a KO mouse |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Chika Saegusa and Mitsunori Fukuda, RIKEN Brain Science Institute in 2006. E14 ES cells derived from 129P2/OlaHsd were used to generated the knockout mice. The mutant mice were crossed C57BL/6. |
Strain description | Slp2 gene knockout mice. Exon 1 of the Slp2 was replaced with a neo cassette. Slp (Synaptoagmin-like protein) is a Rab family of low-molecular-G protein, and known as a molecule implicated in the Rab27-dependent membrane transport. Homozygous mutant mice shows an abnormal gastric surface mucus cell morphology and reduced number of mucus granules. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Decreased basal mucus secretion by Slp2-a-deficient gastric surface mucous cells. Saegusa C, Tanaka T, Tani S, Itohara S, Mikoshiba K, Fukuda M Genes Cells, 11, 623-631 (2006). 16716193Expression of Rab27B-binding protein Slp1 in pancreatic acinar cells and its involvement in amylase secretion. Saegusa C, Kanno E, Itohara S, Fukuda M Arch. Biochem. Biophys., 475(1):87-92 (2008). 18477466 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Sytl2 | synaptotagmin-like 2 | 7 | Sytl2 | targeted mutation 1, Mitsunori Fukuda | |||
neo | neomycin resistance gene (E. coli) | 7 | mouse phosphoglycerate kinase promoter (PGK promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | E. coli neo, mouse PGK promoter, mouse Slp2-a genomic DNA |
Research application | Cell Biology Research Dermatology Research Internal/Organ Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Arch. Biochem. Biophys., 475(1):87-92 (2008). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Permission of Dr. Mitsunori Fukuda is requried before use of the Slp1-KO mice. |
Depositor | Mitsunori Fukuda (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |