Strain Data Sheet

RBRC02078

Strain Information

Image
BRC No.RBRC02078
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Slp2<tm1Mitf>
Former Common nameSlp2-a KO mouse
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Chika Saegusa and Mitsunori Fukuda, RIKEN Brain Science Institute in 2006. E14 ES cells derived from 129P2/OlaHsd were used to generated the knockout mice. The mutant mice were crossed C57BL/6.
Strain descriptionSlp2 gene knockout mice. Exon 1 of the Slp2 was replaced with a neo cassette. Slp (Synaptoagmin-like protein) is a Rab family of low-molecular-G protein, and known as a molecule implicated in the Rab27-dependent membrane transport. Homozygous mutant mice shows an abnormal gastric surface mucus cell morphology and reduced number of mucus granules.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Decreased basal mucus secretion by Slp2-a-deficient gastric surface mucous cells.
Saegusa C, Tanaka T, Tani S, Itohara S, Mikoshiba K, Fukuda M
Genes Cells, 11, 623-631 (2006). 16716193

Expression of Rab27B-binding protein Slp1 in pancreatic acinar cells and its involvement in amylase secretion.
Saegusa C, Kanno E, Itohara S, Fukuda M
Arch. Biochem. Biophys., 475(1):87-92 (2008). 18477466

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Sytl2synaptotagmin-like 27Sytl2targeted mutation 1, Mitsunori Fukuda
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal gastric surface mucous cell morphology(MP:0004137)

  • decreased digestive secretion(MP:0000505)
  • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli neo, mouse PGK promoter, mouse Slp2-a genomic DNA
    Research applicationCell Biology Research
    Dermatology Research
    Internal/Organ Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Arch. Biochem. Biophys., 475(1):87-92 (2008). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Permission of Dr. Mitsunori Fukuda is requried before use of the Slp1-KO mice.
    DepositorMitsunori Fukuda (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data