Strain Data Sheet

RBRC02077

Strain Information

Image
BRC No.RBRC02077
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Sytl1<tm1Mitf>/MitfRbrc
Former Common nameSlp1 KO mouse
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Chika Segusa and Mitsunori Fukuda at RIKEN BSI. A neomysin resistance cassette were inserted into the exon 1 of the Slp1 gene. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionB6;129P2-Sytl1<tm1Mitf>. Slp (Synaptoagmin-like protein) is a Rab family of low-molecular-G protein, and known as a molecule implicated in the Rab27-dependent membrane transport. Homozygous mutant mice shows an increased number of zymogen granules in the pancreatic acinar cells.
Colony maintenance繁殖効率：A
ReferencesArch Biochem Biophys. 2008 Jul 1;475(1):87-92. 18477466

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sytl1synaptotagmin-like 14Sytl1<tm1Mitf>targeted mutation 1, Mitsunori FukudaMGC:7008, PSGL-1, Slp1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4neo; neomycin;

Ordering Information

供与核酸mouse Slp1 genomic DNA, E. coli neo, mouse PGK promoter
Research application
提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
研究成果の公表にあたって寄託者の指定する文献を引用する。Saegusa, C., Kanno E., Itohara S. and Fukuda. M. Expression of Rab27B-binding protein Slp1 in pancreatic acinar cells and its involvement in amylase secretion. Arch Biochem Biophys. 2008 Jul 1;475(1):87-92.
研究成果の公表にあたって謝辞の表明を必要とする。
使用者は事前に寄託者の承諾を得ること。
Depositor福田　光則（独立行政法人理化学研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data