Strain Data Sheet

RBRC02032

Strain Information

Image
BRC No.RBRC02032
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6C3-Tg(CAG/Acr-EGFP)CX-FM139Osb
Former Common nameB6C3F1-Tg(CAG/Acr-EGFP)CX-FM139Osb (Nov. 2011), B6D2F1-Tg(CAG-EGFP)CX-FM139Osb
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a b/b C/C]
Strain developmentDeveloped by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. This transgenic line was generated by coinjected with act-EGFP and acr-EGFP transgene constructs into fertilized eggs. The mice were crossed to C57BL/6.
Strain descriptionTransgenic mice expressing GFP ubiquitously from CAG-EGFP and in the sperm acrosome from acr3-EGFP. GFP expression is detected in germline cells at early stage (8 cells). Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter. CAG-EGFP, in which EGFP is connected to the cytomegalovirus immediate early enhancer/beta-actin promoter (CAG). The transgene is located in chromosome X by FISH.
Colony maintenanceCarrier x Noncarrier [C57BL/6NJcl]
ReferencesGenomics, 80(6):564-574 (2002). 12504848

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)XCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Ordering Information

供与核酸CX-EGFP (Jellyfish GFP cDNA, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, rabbit beta-globin polyadenylation signal
Research applicationFluorescent Proteins/lacZ System
提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
研究成果の公表にあたって寄託者の指定する文献を引用する。Genomics, 80(6):564-574 (2002).
非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること（https://www.ccb.osaka-u.ac.jp/en/）。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。
Depositor岡部　勝（国立大学法人大阪大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data