Strain Data Sheet

RBRC01969

Strain Information

Image
BRC No.RBRC01969
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(CAG-EGFP)1Yam Fbxo15<tm1Yam>
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain development奈良先端科学技術大学院大学・山中伸弥先生（2004）。ICR-Tg(CAG-EGFP)1YamとB6;129S4- Fbxo15<tm1Yam>マウスとの交配により作出。混合背景。
Strain descriptionFbxo15遺伝子のノックアウトとCAG-EGFPトランスジェニックのダブルミュータントマウス系統。
Colony maintenanceTg: Carrier x Noncarrier; KO: Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Efficient selection for high-expression transfectants with a novel eukaryotic vector.
H Niwa, K Yamamura, J Miyazaki
Gene, 108(2):193-9 (1991). 1660837

Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development.
Yoshimi Tokuzawa, Eiko Kaiho, Masayoshi Maruyama, Kazutoshi Takahashi, Kaoru Mitsui, Mitsuyo Maeda, Hitoshi Niwa, Shinya Yamanaka
Mol. Cell. Biol., 23, 2699-2708 (2003). 12665572

Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
Kazutoshi Takahashi, Shinya Yamanaka
Cell, 126, 663-676 (2006). 16904174

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Fbxo15
MGI:1354755		F-box protein 1518Fbxo15<tm1Yam>
MGI:2655776		targeted mutation 1, Shinya Yamanaka
IRES internal ribosomal entry site (EMCV)18
lacZ beta-galactosidase (E. coli)18
neo neomycin resistance gene (E. coli)18
SV40 polyA18
GFP Green Fluorescent Protein (Aequorea victoria)UN CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • no abnormal phenotype detected (MP:0002169)
    • Detailed phenotype data

    Ordering Information

    供与核酸CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), green flourescent protein (jellyfish)、E. coli lacZ, E. coli neo, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), SV40 polyA, mouse Fbxo15 genomic DNA
    Research applicationDevelopmental Biology Research
    提供条件
    Depositor山中　伸弥（国立大学法人京都大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: Japanese (Word) / English(Word)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data