Strain Data Sheet

RBRC01968

Strain Information

Image
BRC No.RBRC01968
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Visfatin<tm1Yam>
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain development開発年：2001年開発者：山中伸弥先生機関名：奈良先端科学技術大学院大学RF8 (129S4/SvJae) ES 細胞をC57BL/6J (日本クレア）のブラストシストに Injectionし、作製。 C57BL/6J（日本クレア）に戻し交配しつつ維持。
Strain descriptionVisfatin is produced by the visceral fat and whose expression level in plasma increases during the development of obesity. Visfatin exterted insulin-mimetic effects in cultured cells and lowered plasma glucose levels in mice. Homozygous mutant mice are embryonic lethal. Heterozygous mice show increased plasma glucose levels but normal insulin evels.
Colony maintenanceHomo 致死のため、　Hetero と　C57BL/6J （日本クレア）で戻し交配しつつ維持。繁殖効率 ： A （良好）
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Namptnicotinamide phosphoribosyltransferase12Nampt<tm1Yam>targeted mutation 1, Shinya Yamanaka1110035O14Rik, Visfatin, Pbef1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)12En2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)12internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)12

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)12neo; neomycin;

Ordering Information

供与核酸En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocorditis virus (EMCV) internal ribosomal entrysite (IRES), E. coli neo, E. coli lacZ, SV40 poly A, mouse Visfatin genomic DNA
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたって謝辞の表明を必要とする。
利用者は、事前に寄託者（連絡先：京都大学 cira-keiyaku@cira.kyoto-u.ac.jp) と覚書を締結する。
Depositor山中　伸弥（国立大学法人京都大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data