Strain Data Sheet

RBRC01967

Strain Information

Image
BRC No.RBRC01967
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Nanog<tm1Yam>
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain development開発年：2003年開発者：山中伸弥先生機関名：奈良先端科学技術大学院大学RF8 (129S4/SvJae) ES 細胞をC57BL/6J (日本クレア）のBlastcysts に Injectionし作製。
Strain descriptionNanog遺伝子はES細胞において万能性を維持する重要な因子の一つである。
Colony maintenanceHomo 致死のため　Hetero と　C57BL/6J （日本クレア）で戻し交配しつつ維持。繁殖効率 ： A （良好）
ReferencesCell, 113, 631-642 (2003). 12787504

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
NanogNanog homeobox6Nanog<tm1Yam>targeted mutation 1, Shinya Yamanaka2410002E02Rik, ecat4, ENK

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)6internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;

Ordering Information

供与核酸mouse Nanog genomic DNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, E. coli neo, SV40 poly A
Research applicationDevelopmental Biology Research
提供条件
Depositor山中　伸弥（国立大学法人京都大学）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: Japanese (Word) / English(Word)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data