Strain Data Sheet

RBRC01966

Strain Information

Image
BRC No.RBRC01966
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-ERas<tm1Yam>
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain development開発年：2003年開発者：山中伸弥先生機関名：奈良先端科学技術大学院大学RF8 (129S4/SvJae) ES 細胞を C57BL/6J (日本クレア） に Injectionし、作製。C57BL/6J（日本クレア） に4回戻し交配し、兄妹交配で Homo 作製維持。
Strain descriptionERas gene is important in the tumor-like grouwth properties of ES cells.Homozygous mutant mice develop normally and are fertile.
Colony maintenanceHomo での維持可能。繁殖効率 ： A （良好）
ReferencesNature, 423, 541-545 (2003). 12774123

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ErasES cell-expressed RasXEras<tm1Yam>targeted mutation 1, Shinya Yamanakaecat5, Ha-Ras2, HRAS2, HRasp

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)Xinternal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)X

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)Xneo; neomycin;

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAX

Ordering Information

供与核酸Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, E. coli neo, SV40 pA, mouse ERas genomic DNA
Research applicationFluorescent Proteins/lacZ System
提供条件
Depositor山中　伸弥（国立大学法人京都大学）
Strain Status凍結胚のアイコン凍結胚
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: Japanese (Word) / English(Word)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data