Strain Data Sheet

RBRC01965

Strain Information

Image
BRC No.RBRC01965
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Fbxo15<tm1Yam>
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain development開発年：2003年開発者：山中伸弥先生機関名：奈良先端科学技術大学院大学RF8 (129S4/SvJae) ES 細胞を、C57BL/6J (日本クレア） の Blastcysts に Injection し作製。 C57BL/6J （日本クレア）に数回戻し交配し、兄妹交配で Homo 作製、維持。
Strain descriptioniPS細胞 (誘導性多能性幹細胞 induced pluripotent stem cell) 作製系統。
Colony maintenanceHomo での維持可能。繁殖効率 ： A （良好）
ReferencesMol. Cell. Biol., 23, 2699-2708 (2003). 12665572

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Fbxo15F-box protein 1518Fbxo15<tm1Yam>targeted mutation 1, Shinya Yamanakaecat3, Fbx15

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)18internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)18

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)18neo; neomycin;

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA18

Ordering Information

供与核酸Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, E. coli neo, SV40 poly A, mouse Fbxo15 genomic DNA
Research applicationDevelopmental Biology Research
提供条件
Depositor山中　伸弥（国立大学法人京都大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: Japanese (Word) / English(Word)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Donato V, Bonora M, Simoneschi D, Sartini D, Kudo Y, Saraf A, Florens L, Washburn MP, Stadtfeld M, Pinton P, Pagano M.
The TDH-GCN5L1-Fbxo15-KBP axis limits mitochondrial biogenesis in mouse embryonic stem cells.
Nat Cell Biol 19(4) 341-351(2017) 28319092