Strain Data Sheet

RBRC01953

Strain Information

Image
BRC No.RBRC01953
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pax1<tm1(LHED)Jtak>
Former Common namePax1LHED
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain development2001年竹田潤二先生大阪大学先端科学イノベーションセンターバイオ・生命系分野C57BL 6 X 129 SvJae の mixed background, 世代数は不明。
Strain descriptionSleeping Beauty トランスポゾンシステムに基づく　Local Hopping Enhancer Detection (LHED) ベクターを Pax1 遺伝子座の 50kb 上流のゲノム領域に相同組み替えによりノックインしたもの。
Colony maintenance繁殖効率 ：A
References
A transposon-based chromosomal engineering method to survey a large cis-regulatory landscape in mice.
Kokubu C, Horie K, Abe K, Ikeda R, Mizuno S, Uno Y, Ogiwara S, Ohtsuka M, Isotani A, Okabe M, Imai K, Takeda J
Nat. Genet., 41:946-952 (2009). 19633672

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site2Frt
Pax1
MGI:97485		paired box 12Pax1<tm1(LHED)Jtak> targeted mutation 1 (LHED), Junji Takeda
  • otofaciocervical syndrome 2(MedGEN)
    • lacZ beta-galactosidase (E. coli)2
    loxP phage P1 loxP2loxP
    neo neomycin resistance gene (E. coli)2 mouse phosphoglycerate kinase promoter (PGK promoter)
    tetO tetracycline operator (E. coli)2tetO

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • no abnormal phenotype detected (MP:0002169)
    • Detailed phenotype data

    Ordering Information

    供与核酸Mouse Phosphoglycerate kinase promoter, Mouse Hsp68 promoter, E. coli LacZ gene,E. coli Neomycin resistance gene, E. coli Tetracycline operator, Fish Sleeping Beauty transposon (IR/DR), P1 phage LoxP, Yeast FRT
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Tet system
    提供条件利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。Nat. Genet., 41:946-952 (2009).非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。
    Depositor竹田　潤二（国立大学法人大阪大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Takeda Lab HP, Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data