Strain Data Sheet

RBRC01948

Strain Information

Image
BRC No.RBRC01948
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Mesp2<tm13(cre/Esr1*)Ysa>/Rbrc
Former Common nameP2MCM; P2MCM knock-in mouse; P2neoMCM knock-in
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development2004年、安彦 行人先生ら、国立医薬品食品衛生研究所ES 細胞 (TT2) を用いた遺伝子ターゲティングにより本機関で樹立。ICR 系マウスの交配 (N= )
Strain description
Colony maintenanceICR 系マウスにて繁殖良好。 ホモは致死。
References
Appropriate suppression of Notch signaling by Mesp factors is essential for stripe pattern formation leading to segment boundary formation.
Takahashi Y, Yasuhiko Y, Kitajima S, Kanno J, Saga Y
Dev Biol., 304, 593-603 (2007). 17306789

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Esr1* estrogen receptor 1 (alpha), mouse ER with a mutation G525R)7Esr1*
Frt yeast FRT (flippase recombination target) site7Frt
Frt yeast FRT (flippase recombination target) site7Frt
Mesp2
MGI:1096325
mesoderm posterior 27Mesp2<tm13(cre/Esr1*)Ysa>
MGI:3715268
targeted mutation 13, Yumiko Saga
  • spondylocostal dysostosis 1, autosomal recessive(MedGEN)

  • spondylocostal dysostosis 2, autosomal recessive(MedGEN)
  • cre Phage P1 Cre recombinase7cre
    neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal somite development(MP:0001688)

  • abnormal vertebrae morphology(MP:0000137)
  • Detailed phenotype data

    Ordering Information

    供与核酸mutant-type mouse estrogen receptor (Mer) cDNA, Phage P1 Cre recombinase, yeast FRT (flippase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, polyA, mouse Mesp2 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。Dev. Biol., 304, 593-603 (2007).
    Depositor菅野 純(国立医薬品食品衛生研究所)
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data