Strain Data Sheet

RBRC01948

Strain Information

Image
BRC No.RBRC01948
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Mesp2<tm13(cre/Esr1*)Ysa>/Rbrc
Former Common nameP2MCM; P2MCM knock-in mouse; P2neoMCM knock-in
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development2004年、安彦　行人先生ら、国立医薬品食品衛生研究所ES 細胞 (TT2) を用いた遺伝子ターゲティングにより本機関で樹立。ICR 系マウスの交配 (N= )
Strain description
Colony maintenanceICR 系マウスにて繁殖良好。　ホモは致死。
ReferencesDev Biol., 304, 593-603 (2007). 17306789

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mesp2mesoderm posterior 27Mesp2<tm13(cre/Esr1*)Ysa>targeted mutation 13, Yumiko Saga

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Esr1*estrogen receptor 1 (alpha), mouse ER with a mutation G525R)7Esr1*

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase7cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

供与核酸mutant-type mouse estrogen receptor (Mer) cDNA, Phage P1 Cre recombinase, yeast FRT (flippase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, polyA, mouse Mesp2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Dev. Biol., 304, 593-603 (2007).
Depositor菅野　純（国立医薬品食品衛生研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data