Strain Data Sheet

RBRC01942

Strain Information

Image
BRC No.RBRC01942
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S2-Creb3l4<tm1Noj>
Former Common nameTisp40 KO mouse
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Norikazu Yabuta and Hiroshi Nojima, Research Institute for Microbial Diseases, Osaka University in 2004. D3 ES cells were used. The mutant mice were crossed to C57BL/6 over 6 times.
Strain descriptionTISP40 (Creb3l4) gene knockout mice. Exons 5 to 10 were replaced with a neo cassette. Homozygous mutant mice are viable and fertile.
Colony maintenanceHeterozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
References
The testes-specific bZip type transcription factor Tisp40 plays a role in ER stress responses and chromatin packaging during spermiogenesis.
Nagamori I, Yomogida K, Ikawa M, Okabe M, Yabuta N, Nojima H
Genes Cells, 11, 1161-1171 (2006). 16999736

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Creb3l4cAMP responsive element binding protein 3-like 43Creb3l4targeted mutation 1, Hiroshi Nojima
neoneomycin resistance gene (E. coli)3mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal spermatid morphology(MP:0006380)

  • decreased male germ cell number(MP:0004901)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Creb3l4 genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo
    Research applicationCell Biology Research
    Reproductive Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes to Cells, 11, 1161-1171 (2006).The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorHiroshi Nojima (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Qin C, Li M, Bai T, Yang K, Xu T, Zhang J.
    Tisp40 deficiency limits renal inflammation and promotes tubular cell proliferation in renal ischemia reperfusion injury.
    Exp Cell Res 371(1) 255-261(2018) 30121191
    Qin C, Xiao C, Su Y, Zheng H, Xu T, Lu J, Luo P, Zhang J.
    Tisp40 deficiency attenuates renal ischemia reperfusion injury induced apoptosis of tubular epithelial cells.
    Exp Cell Res 359(1) 138-144(2017) 28778797