Strain Data Sheet

RBRC01941

Strain Information

Image
BRC No.RBRC01941
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Nod2<tm1Jhgt>/GvnRbrc
Former Common nameCard15 KO (129/Ola background), 129-Card15<tm1>/GvnRbrc
H-2 Haplotype
ES Cell line
Background strain129
Appearance
Strain developmentDeveloped by Marco Giovannini, INSERM. PGK-hygromycin cassette was removed by cre recombinase.
Strain descriptionThis mouse was produced from the knock-in targeting of Card15 (Nod2) locus with EGFP cassette. The expression of the GFP is relatively low. C57BL/6 congenic (RBRC01869), C57BL/6 and 129 mixed background (RBRC01941).
Colony maintenanceHomozygote x Heterozygote, Homozygote
References
CARD15/NOD2 is required for Peyer's patches homeostasis in mice.
Barreau F, Meinzer U, Chareyre F, Berrebi D, Niwa-Kawakita M, Dussaillant M, Foligne B, Ollendorff V, Heyman M, Bonacorsi S, Lesuffleur T, Sterkers G, Giovannini M, Hugot J P
PLoS One, 2(6), e523 (2007). 17565376

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)8
  • Blau syndrome(MedGEN)

  • Crohn disease(DisGeNET)

  • inflammatory bowel disease 1(MedGEN)
  • more 1 Diseases
  • Yao syndrome(MedGEN)
  • HBB2rabbit beta-globin polyA8HBB2
  • Blau syndrome(MedGEN)

  • Crohn disease(DisGeNET)

  • inflammatory bowel disease 1(MedGEN)
  • more 1 Diseases
  • Yao syndrome(MedGEN)
  • Nod2nucleotide-binding oligomerization domain containing 28Nod2
  • Blau syndrome(MedGEN)

  • Crohn disease(DisGeNET)

  • inflammatory bowel disease 1(MedGEN)
  • more 1 Diseases
  • Yao syndrome(MedGEN)
  • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal Peyer's patch morphology(MP:0000696)

  • abnormal cytokine level(MP:0008713)

  • abnormal immune system morphology(MP:0000685)

  • abnormal immune system physiology(MP:0001790)

  • abnormal interferon level(MP:0008750)
  • more 9 phenotypes
  • abnormal interleukin level(MP:0008751)

  • abnormal lymph node B cell domain morphology(MP:0002344)

  • abnormal response to infection(MP:0005025)

  • abnormal tumor necrosis factor level(MP:0008752)

  • enlarged Peyer's patches(MP:0008136)

  • increased CD4-positive, alpha-beta T cell number(MP:0008074)

  • increased Peyer's patch number(MP:0008132)

  • increased double-negative T cell number(MP:0005090)

  • increased susceptibility to induced colitis(MP:0008537)
  • Detailed phenotype data

    Ordering Information

    Donor DNACard 15 exon1 Knock-in EGFP, Rabbit beta-globin poly A
    Research applicationInternal/Organ Research
    Mouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT shall keep DEPOSITOR apprised of any results obtained from the BIOLOGICAL RESOURCE in the course of the research, as soon as they are available. Moreover, if the research results in a potential invention or a substance that may have potential commercial value (hereinafter referred as to the "Invention") RECIPIENT will promptly disclose this Invention to DEPOSITOR with samples thereof for DEPOSITOR's internal use.
    RECIPIENT and DEPOSITOR will consult together and agree about the possibility of filing one or more patent application to Invention conceived by one or more employees of Recipients that directly arise out of the research. The Parties will negotiate in good faith the conditions of such patent application according to each party's inventiveness contribution in the Invention.
    In case of such Invention, patentable or not, reduced in practice or developed in the course of the research that could not have been obtained, reduced to practice or developed but from using the Material and/or Confidential Information, RECIPIENT and DEPOSITOR shall confer with one another as regards licensing and commercialization of such Invention to negotiate in good faith the terms of an institutional agreement as regards said matters.
    DepositorMarco Giovannini (INSERM)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data