Strain Data Sheet
RBRC01939
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC01939 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129X1-Trpv4<tm1Msz> |
| Former Common name | TRPV4KO |
| H-2 Haplotype | |
| ES Cell line | RW4 [129X1/SvJ] |
| Background strain | C57BL/6JJcl |
| Appearance | black [a/a b/b C/C] |
| Strain development | 自治医科大学・水野 誠先生（2001）。RW4 ES細胞を用いて作出。C57BL/6へ戻し交配された。 |
| Strain description | TRPV4遺伝子のノックアウトマウス。Trpv4 (transient receptor potential cation channel, subfamily V, member 4) は機械刺激や温度を感受してCa<2+>を通すチャネルである。ホモノックアウトマウスは、生存性、妊性ともに正常であるが、浸透圧感受、圧感受、温度感受の低下や、すり応力、痛みや刺激の反応低下などが、様々な臓器で報告されている。 |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
| References | J. Biol. Chem., 278, 22664-22668 (2003). 12692122 Am. J. Physiol. Cell Physiol., 285, C96-C101(2003). 12777254 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2024/01/09Room:3-ARack:I 2023/10/10Room:3-ARack:I 2023/07/10Room:3-ARack:I 2023/04/10Room:3-ARack:I 2023/01/10Room:3-ARack:I 2022/10/11Room:3-ARack:I 2022/07/11Room:3-ARack:I |
Gene | |
|---|---|
| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Trpv4transient receptor potential cation channel, subfamily V, member 45Trpv4<tm1Msz>targeted mutation 1, Makoto Suzuki0610033B08Rik, OTRPC4, Trp12, VR-OAC, VRL-2, VROAC Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)5neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
|---|---|
| 供与核酸 | mouse Trpv4 genomic DNA, PGK-neo cassette (mouse phosphoglycerate kinase-1 promoter, E. coli aminoglycoside phosphotransferase cDNA)derived from the plasmid pKJ2. |
| Research application | Sensorineural Research |
| 提供条件 | 条件を付加する。 研究成果の公表にあたって寄託者の指定する文献を引用する。J, Biol. Chem., 278, 22664-22668 (2003). Am. J. Physiol. Cell Physiol., 285, C96-C101(2003). |
| Depositor | 鈴木 誠（自治医科大学） |
| Strain Status |  生体 凍結胚 凍結精子 |
| Strain Availability | 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 生体マウスを1~3ヶ月以内に提供可能 |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
|---|
Ueda T, Hoshikawa M, Shibata Y, Kumamoto N, Ugawa S. Basal cells express functional TRPV4 channels in the mouse nasal epithelium. Biochem Biophys Rep 4 169-174(2015) 29124201 Weber J, Rajan S, Schremmer C, Chao YK, Krasteva-Christ G, Kannler M, Yildirim AÖ, Brosien M, Schredelseker J, Weissmann N, Grimm C, Gudermann T, Dietrich A. TRPV4 channels are essential for alveolar epithelial barrier function as protection from lung edema. JCI Insight 5(20) (2020) 32931478 |