Strain Data Sheet

RBRC01939

Strain Information

Image
BRC No.RBRC01939
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129X1-Trpv4<tm1Msz>
Former Common nameTRPV4KO
H-2 Haplotype
ES Cell lineRW4 [129X1/SvJ]
Background strainC57BL/6JJcl
Appearanceblack [a/a b/b C/C]
Strain developmentDeveloped by Makoto Suzuki, Jichi Medical School in 2001. The construct was electoroporated into RW4 ES cells derived from 129X1/SvJ. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionTRPV4 (Trpv4, transient receptor potential cation channel, subfamily V, member 4) knockout mice. Exon 4 encodes the ankyrin-repeat domain was replaced with a neomycin resistance gene. The ion channel TRPV4 is a member of the TRP family, acts as a Ca2+-permeable cation channel in response to mechanical stress. Homozygous mutant mice are viable and fertile, but show a reduced responsiveness to pressure sensation and abnormal osmotic regulation.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Impaired pressure sensation in mice lacking TRPV4.
Makoto Suzuki, Atsuko Mizuno, Kunihiko Kodaira, Masashi Imai
J. Biol. Chem., 278, 22664-22668 (2003). 12692122

Impaired osmotic sensation in mice lacking TRPV4.
Atsuko Mizuno, Naoko Matsumoto, Masashi Imai, Makoto Suzuki
Am. J. Physiol. Cell Physiol., 285, C96-C101(2003). 12777254

Health Report

Examination Date / Room / Rack2025/11/10Room:3-ARack:ISentinel mouse program
2025/09/01Room:3-ARack:ISentinel mouse program
2025/06/16Room:3-ARack:ISentinel mouse program
2025/04/07Room:3-ARack:ISentinel mouse program
2025/01/07Room:3-ARack:ISentinel mouse program
2024/07/08Room:3-ARack:ISentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Trpv4
MGI:1926945		transient receptor potential cation channel, subfamily V, member 45Trpv4<tm1Msz>
MGI:2667379		targeted mutation 1, Makoto Suzuki
  • autosomal dominant brachyolmia(MedGEN)

  • avascular necrosis of femoral head, primary, 2(MedGEN)

  • Charcot-Marie-Tooth disease axonal type 2C(MedGEN)
    • more 7 Diseases
  • familial digital arthropathy-brachydactyly(MedGEN)

  • metatropic dysplasia(DisGeNET, MedGEN)

  • neuronopathy, distal hereditary motor, autosomal dominant 8(MedGEN)

  • parastremmatic dwarfism(MedGEN)

  • scapuloperoneal spinal muscular atrophy, autosomal dominant(MedGEN)

  • spondyloepimetaphyseal dysplasia, Maroteaux type(MedGEN)

  • spondylometaphyseal dysplasia, Kozlowski type(MedGEN)
    • neo neomycin resistance gene (E. coli)5 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal response to tactile stimuli (MP:0005316)

  • abnormal sensory capabilities/reflexes/nociception (MP:0002067)

  • impaired hearing (MP:0006325)

  • increased chemical nociceptive threshold (MP:0008531)

  • increased or absent threshold for auditory brainstem response (MP:0011967)
    • more 1 phenotypes
  • increased susceptibility to noise-induced hearing loss (MP:0004597)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Trpv4 genomic DNA, PGK-neo cassette (mouse phosphoglycerate kinase-1 promoter, E. coli aminoglycoside phosphotransferase cDNA)derived from the plasmid pKJ2.
    Research applicationSensorineural Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J, Biol. Chem., 278, 22664-22668 (2003). Am. J. Physiol. Cell Physiol., 285, C96-C101(2003).
    DepositorMakoto Suzuki (Jichi Medical University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Alt P, Müller I, Kiefmann M, Gudermann T, Kuebler WM, Griese M, Staab-Weijnitz CA, Dietrich A.
    Transient receptor potential vanilloid 4 (TRPV4) channels mediate pulmonary surfactant protein A and D secretion.
    Am J Respir Cell Mol Biol (2026) 41738221
    Zhou T, Wang Z, Lv X, Guo M, Zhang N, Liu L, Geng L, Shao J, Zhang K, Gao M, Mao A, Zhu Y, Yu F, Feng L, Wang X, Zhai Q, Chen W, Ma X.
    Targeting gut S. aureofaciens Tü117 serves as a new potential therapeutic intervention for the prevention and treatment of hypertension.
    Cell Metab 37(2) 496-513.e11(2025) 39908987
    Schaller L, Gudermann T, Dietrich A.
    TRPV4 Mediates Alveolar Epithelial Barrier Integrity and Induces ADAM10-Driven E-Cadherin Shedding.
    Cells 13(20) (2024) 39451235
    Murakami D, Kono M, Sakatani H, Iyo T, Hijiya M, Shiga T, Kinoshita T, Sumioka T, Okada Y, Saika S, Koizumi Y, Hotomi M.
    Inhibition of transient receptor potential vanilloid 1 reduces shedding and transmission during Streptococcus pneumoniae co-infection with influenza.
    Infect Immun 92(10) e0014624(2024) 39109830
    Sakatani H, Kono M, Shiga T, Kuwazoe H, Nanushaj D, Matsuzaki I, Murata SI, Miyajima M, Okada Y, Saika S, Hotomi M.
    The Roles of Transient Receptor Potential Vanilloid 1 and 4 in Olfactory Regeneration.
    Lab Invest 103(4) 100051(2023) 36870285
    Sanjel B, Kim BH, Song MH, Carstens E, Shim WS.
    Glucosylsphingosine evokes pruritus via activation of 5-HT2A receptor and TRPV4 in sensory neurons.
    Br J Pharmacol 179(10) 2193-2207(2022) 34766332
    Kono M, Nanushaj D, Sakatani H, Murakami D, Hijiya M, Kinoshita T, Shiga T, Kaneko F, Enomoto K, Sugita G, Miyajima M, Okada Y, Saika S, Hotomi M.
    The Roles of Transient Receptor Potential Vanilloid 1 and 4 in Pneumococcal Nasal Colonization and Subsequent Development of Invasive Disease.
    Front Immunol 12 732029(2021) 34804016
    Lee WJ, Shim WS.
    Cutaneous Neuroimmune Interactions of TSLP and TRPV4 Play Pivotal Roles in Dry Skin-Induced Pruritus.
    Front Immunol 12 772941(2021) 34925342
    Weber J, Rajan S, Schremmer C, Chao YK, Krasteva-Christ G, Kannler M, Yildirim AÖ, Brosien M, Schredelseker J, Weissmann N, Grimm C, Gudermann T, Dietrich A.
    TRPV4 channels are essential for alveolar epithelial barrier function as protection from lung edema.
    JCI Insight 5(20) (2020) 32931478
    Robinson RK, Birrell MA, Adcock JJ, Wortley MA, Dubuis ED, Chen S, McGilvery CM, Hu S, Shaffer MSP, Bonvini SJ, Maher SA, Mudway IS, Porter AE, Carlsten C, Tetley TD, Belvisi MG.
    Mechanistic link between diesel exhaust particles and respiratory reflexes.
    J Allergy Clin Immunol 141(3) 1074-1084.e9(2018) 28532657
    Okada Y, Shirai K, Miyajima M, Reinach PS, Yamanaka O, Sumioka T, Kokado M, Tomoyose K, Saika S.
    Loss of TRPV4 Function Suppresses Inflammatory Fibrosis Induced by Alkali-Burning Mouse Corneas.
    PLoS One 11(12) e0167200(2016) 28030558
    Ueda T, Hoshikawa M, Shibata Y, Kumamoto N, Ugawa S.
    Basal cells express functional TRPV4 channels in the mouse nasal epithelium.
    Biochem Biophys Rep 4 169-174(2015) 29124201