Strain Information | |
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Image | |
BRC No. | RBRC01939 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129X1-Trpv4<tm1Msz> |
Former Common name | TRPV4KO |
H-2 Haplotype | |
ES Cell line | RW4 [129X1/SvJ] |
Background strain | C57BL/6JJcl |
Appearance | black [a/a b/b C/C] |
Strain development | Developed by Makoto Suzuki, Jichi Medical School in 2001. The construct was electoroporated into RW4 ES cells derived from 129X1/SvJ. The mutant mice were backcrossed to C57BL/6J. |
Strain description | TRPV4 (Trpv4, transient receptor potential cation channel, subfamily V, member 4) knockout mice. Exon 4 encodes the ankyrin-repeat domain was replaced with a neomycin resistance gene. The ion channel TRPV4 is a member of the TRP family, acts as a Ca2+-permeable cation channel in response to mechanical stress. Homozygous mutant mice are viable and fertile, but show a reduced responsiveness to pressure sensation and abnormal osmotic regulation. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Impaired osmotic sensation in mice lacking TRPV4. Mizuno A, Matsumoto N, Imai M, Suzuki M Am. J. Physiol. Cell Physiol., 285, C96-C101(2003). 12777254Impaired pressure sensation in mice lacking TRPV4. Suzuki M, Mizuno A, Kodaira K, Imai M J. Biol. Chem., 278, 22664-22668 (2003). 12692122 |
Health Report | |
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Examination Date / Room / Rack | 2024/10/07Room:3-ARack:I 2024/07/08Room:3-ARack:I 2024/04/08Room:3-ARack:I 2024/01/09Room:3-ARack:I 2023/10/10Room:3-ARack:I 2023/07/10Room:3-ARack:I 2023/04/10Room:3-ARack:I 2023/01/10Room:3-ARack:I |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Trpv4 | transient receptor potential cation channel, subfamily V, member 4 | 5 | Trpv4 | targeted mutation 1, Makoto Suzuki | more 7 Diseases | ||
neo | neomycin resistance gene (E. coli) | 5 | mouse phosphoglycerate kinase promoter (PGK promoter) | more 7 Diseases |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 1 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | mouse Trpv4 genomic DNA, PGK-neo cassette (mouse phosphoglycerate kinase-1 promoter, E. coli aminoglycoside phosphotransferase cDNA)derived from the plasmid pKJ2. |
Research application | Sensorineural Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J, Biol. Chem., 278, 22664-22668 (2003). Am. J. Physiol. Cell Physiol., 285, C96-C101(2003). |
Depositor | Makoto Suzuki (Jichi Medical University) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Ueda T, Hoshikawa M, Shibata Y, Kumamoto N, Ugawa S. Basal cells express functional TRPV4 channels in the mouse nasal epithelium. Biochem Biophys Rep 4 169-174(2015) 29124201 |
Sanjel B, Kim BH, Song MH, Carstens E, Shim WS. Glucosylsphingosine evokes pruritus via activation of 5-HT2A receptor and TRPV4 in sensory neurons. Br J Pharmacol 179(10) 2193-2207(2022) 34766332 |
Schaller L, Gudermann T, Dietrich A. TRPV4 Mediates Alveolar Epithelial Barrier Integrity and Induces ADAM10-Driven E-Cadherin Shedding. Cells 13(20) (2024) 39451235 |
Lee WJ, Shim WS. Cutaneous Neuroimmune Interactions of TSLP and TRPV4 Play Pivotal Roles in Dry Skin-Induced Pruritus. Front Immunol 12 772941(2021) 34925342 |
Weber J, Rajan S, Schremmer C, Chao YK, Krasteva-Christ G, Kannler M, Yildirim AÖ, Brosien M, Schredelseker J, Weissmann N, Grimm C, Gudermann T, Dietrich A. TRPV4 channels are essential for alveolar epithelial barrier function as protection from lung edema. JCI Insight 5(20) (2020) 32931478 |