Strain Information | |
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Image | |
BRC No. | RBRC01926 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129S4-Vamp7<tm1Aha> |
Former Common name | Vamp7 KO, B6;129-Vamp7<tm1> |
H-2 Haplotype | |
ES Cell line | J1 [129S4/SvJae] |
Background strain | |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Akihiro Harada, Institute for Molecular and Cellular Regulation, Gunma University in 2005. Two lines were generated (RBRC01926 and RBRC04855). |
Strain description | Vamp7 floxed mice (Neo type). Exons 3 and 4 of the Vamp7 gene were flanked by loxP sites, an FRT-SA-Ex 5,6,7,8-IRES-Neo-polyA-FRT cassette was inserted between exons 4 and 5. Vamp7 is a member of VAMP (vesicle-associated membrane protein) subfamily of SNARES proteins which primarily mediate fusion of cellular transport vesicles with a target membrane, and ubiquitously expressed and in localized to the late endsome, the lysosome, and the trans-Golgi network (TGN). |
Colony maintenance | Homozygote x HemizygoteHomozgous females and hemizygous males are viable and fertile. |
References | Role of tetanus neurotoxin insensitive vesicle-associated membrane protein (TI-VAMP) in vesicular transport mediating neurite outgrowth. Martinez-Arca S, Alberts P, Zahraoui A, Louvard D, Galli T J. Cell Biol., 2000 May 15;149(4):889-900. 10811829The role of VAMP7/TI-VAMP in cell polarity and lysosomal exocytosis in vivo. Sato M, Yoshimura S, Hirai R, Goto A, Kunii M, Atik N, Sato T, Sato K, Harada R, Shimada J, Hatabu T, Yorifuji H, Harada A Traffic., 12(10):1383-1393 (2011). 21740490A novel tetanus neurotoxin-insensitive vesicle-associated membrane protein in SNARE complexes of the apical plasma membrane of epithelial cells. Galli T, Zahraoui A, Vaidyanathan V V, Raposo G, Tian J M, Karin M, Niemann H, Louvard D Mol. Biol. Cell, 9, 1437-1448 (1998). 9614185 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Frt | yeast FRT (flippase recombination target) site | X | Frt | ||||
Frt | yeast FRT (flippase recombination target) site | X | Frt | ||||
IRES | internal ribosomal entry site (EMCV) | X | |||||
Vamp7 | vesicle-associated membrane protein 7 | X | Vamp7 | targeted mutation 1, Akihiro Harada | |||
loxP | phage P1 loxP | X | loxP | ||||
loxP | phage P1 loxP | X | loxP | ||||
neo | neomycin resistance gene (E. coli) | X |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli neo, phage P1 loxP sites, yeast FRT (flipase recombination target) sites, mouse VAMP7 genomic DNA |
Research application | Cell Biology Research Cre/loxP system FLP/frt system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Within a period of TWO years after the first paper concerning the analysis of the BIOLOGICAL RESOURCE is published, the RECIPIENT agree to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. |
Depositor | Akihiro Harada (Gunma University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Xu H, Oses-Prieto JA, Khvotchev M, Jain S, Liang J, Burlingame A, Edwards RH. Adaptor protein AP-3 produces synaptic vesicles that release at high frequency by recruiting phospholipid flippase ATP8A1. Nat Neurosci (2023) 37723322 |