Strain Data Sheet

RBRC01886

Strain Information

Image
BRC No.RBRC01886
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.Cg-Aff3<Tn(sb-lacZ,GFP)PV516Jtak>/JtakRbrc
Former Common nameGene trap of Laf4 (lymphoid nuclear protein related to AF4), PV516
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Junji Takeda at Osaka University in 2005.
Strain descriptionThis strain is a gene trap strain that have an EGFP in Smoc1 gene, produced by using the SB transposon system.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
References
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Horie K, Yusa K, Yae K, Odajima J, Fischer S E, Keng V W, Hayakawa T, Mizuno S, Kondoh G, Ijiri T, Matsuda Y, Plasterk R H, Takeda J
Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530

Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system.
Keng V W, Yae K, Hayakawa T, Mizuno S, Uno Y, Yusa K, Kokubu C, Kinoshita T, Akagi K, Jenkins N A, Copeland N G, Horie K, Takeda J
Nat. Methods, 2, 763-769 (2005). 16179923

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aff3AF4/FMR2 family, member 31Aff3gene trap trap lacZ/GFP PV384, Junji Takeda
GFPGreen Fluorescent Protein (Aequorea victoria)1CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
Hprtmouse Hprt splice donor (SD)1Hprt

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAsalmonid Sleeping Beauty transposon, mouse Hprt splice donor, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), jellyfish GFP cDNA, polyA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorJunji Takeda (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Takeda Lab HP, Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data