Strain Data Sheet

RBRC01883

Strain Information

Image
BRC No.RBRC01883
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.Cg-Sox5<Tn(sb-lacZ,GFP)OR123Jtak>/JtakRbrc
Former Common nameGene trap of Sox5 (SRY-box containing gene 5), OR132
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Junji Takeda at Osaka University in 2005.
Strain description
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
References
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Horie K, Yusa K, Yae K, Odajima J, Fischer S E, Keng V W, Hayakawa T, Mizuno S, Kondoh G, Ijiri T, Matsuda Y, Plasterk R H, Takeda J
Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530

Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system.
Keng V W, Yae K, Hayakawa T, Mizuno S, Uno Y, Yusa K, Kokubu C, Kinoshita T, Akagi K, Jenkins N A, Copeland N G, Horie K, Takeda J
Nat. Methods, 2, 763-769 (2005). 16179923

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)6CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
  • Lamb-Shaffer syndrome(MedGEN)
    • IRESinternal ribosomal entry site (EMCV)6
  • Lamb-Shaffer syndrome(MedGEN)
    • SASplice acceptor (adenovirus)6SA
  • Lamb-Shaffer syndrome(MedGEN)
    • Sox5SRY (sex determining region Y)-box 56Sox5gene trap trap lacZ/GFP OR132, Junji Takeda
  • Lamb-Shaffer syndrome(MedGEN)
    • Splice donorSplice donor (mouse hprt)6Splice donor
  • Lamb-Shaffer syndrome(MedGEN)
    • lacZbeta-galactosidase (E. coli)6
  • Lamb-Shaffer syndrome(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • cyanosis(MP:0001575)

  • neonatal lethality, complete penetrance(MP:0011087)

  • respiratory distress(MP:0001954)
    • Detailed phenotype data

    Ordering Information

    Donor DNAサケ由来 Sleeping Beauty トランスポゾン, サケ由来 Sleeping Beauty トランスポゼース, GFP 遺伝子, LacZ 遺伝子, neo 遺伝子,Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires),Human bcl-2 スプライスアクセプター, Mouse hprt スプライスドナー, Cytomegalovirus エンハンサー, Chicken beta-actin プロモーター, Mouse pgk プロモーター, Rabbit beta-globin ポリA付加シグナル, Mouse synaptonemal protein 1 プロモーター, Human growth hormone ポリ A付加シグナル, pBluescript
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorJunji Takeda (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Takeda Lab HP, Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data