Strain Information | |
---|---|
Image | |
BRC No. | RBRC01882 |
Type | Gene Trap |
Species | Mus musculus |
Strain name | B6.Cg-Arpc3<Tn(sb-lacZ,GFP)T5,117Jtak> |
Former Common name | Gene trap of arpc3 (Arp2/3 complex subunit 3), TM117 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Dr. Junji Takeda, Graduate School of Medicine, Osaka University in 2005. |
Strain description | This strain is a gene trap strain that have an EGFP in Arpc3 gene, produced by using the SB transposon system. |
Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl) |
References | Nat. Methods, 2, 763-769 (2005). 16179923 Mol. Cell. Biol., 26, 6185-6196 (2006). 16880528 Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Arpc3actin related protein 2/3 complex, subunit 35Arpc3<Tn(sb-lacZ,GFP)T5,117Jtak>transposon insertion T5.117, Junji Takeda1110006A04Rik, Arp2/3 complex subunit p21-Arc, p21-Ar Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)5CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)5internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor (adenovirus)5SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Splice donorSplice donor (mouse hprt)5Splice donor Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)5 |
Ordering Information | |
---|---|
Donor DNA | サケ由来 Sleeping Beauty トランスポゾン, サケ由来 Sleeping Beauty トランスポゼース, GFP 遺伝子, LacZ 遺伝子, neo 遺伝子,Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires),Human bcl-2 スプライスアクセプター, Mouse hprt スプライスドナー, Cytomegalovirus エンハンサー, Chicken beta-actin プロモーター, Mouse pgk プロモーター, Rabbit beta-globin ポリA付加シグナル, Mouse synaptonemal protein 1 プロモーター, Human growth hormone ポリ A付加シグナル, pBluescript |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. |
Depositor | Junji Takeda (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |