Strain Data Sheet

RBRC01862

Strain Information

Image
BRC No.RBRC01862
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Mesp2<tm(GFP)>/YsaRbrc
Former Common nameMesp2-GFP
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development2006年、相賀裕美子先生、国立遺伝研MCHに２-3回back cross３世代
Strain descriptionMesp2の変わりにGFPが発現する。
Colony maintenanceヘテロで維持形態の異常・奇形がある。ホモは致死、脊椎骨異常。
ReferencesDev. Biol., 300, 687-698 (2006). 16996494

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mesp2mesoderm posterior 27Mesp2<tm11Ysa>targeted mutation 11, Yumiko Saga

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)7

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)7GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

供与核酸Jellyfish GFP cDNA, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, bovine GH polyA, mouse mesp2 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Dev. Biol., 300, 687-698 (2006).
Depositor相賀　裕美子（大学共同利用機関法人情報・システム研究機構国立遺伝学研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Saga Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Lauschke VM, Tsiairis CD, François P, Aulehla A.
Scaling of embryonic patterning based on phase-gradient encoding.
Nature 493(7430) 101-5(2013) 23254931