Strain Data Sheet

RBRC01860

Strain Information

Image
BRC No.RBRC01860
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameFVB-Tg(EF1-Invs*dC/EGFP)N105Hmd
Former Common nameInv Delta C::GFP(N105)
H-2 Haplotype
ES Cell line
Background strainFVB/N
Appearance
Strain developmentDeveloped by Drs. Yukio Saijho and Hiroshi Hamada, Institute for Molecular and Cellular Biology, Osaka University at 2000. The transgene was injected into the pronuclei of FVB/N fertilized eggs.
Strain descriptionThis transgenic strain expresses the mouse Invs cDNA and GFP fusion gene under the control of the EF1alpha gene promoter. Inv::GFP type (line B27): the coding region of the mouse Invs cDNA was linked to EGFP (RBRC01859), Inv deltaC::GFP (line N105): deleted the 3’ part of the coding region of the Inv cDNA at the NheI site (RBRC01860). These strains are useful for elucidation of the function of Invs gene.
Colony maintenanceCarrier x Noncarrier [or Crossing to FVB/NJcl]
References
The left-right determinant Inversin is a component of node monocilia and other 9+0 cilia.
Watanabe D, Saijoh Y, Nonaka S, Sasaki G, Ikawa Y, Yokoyama T, Hamada H
Development, 130, 1725-1734 (2003). 12642479

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)UN
  • nephronophthisis 2(DisGeNET, MedGEN)
    • InvsInversinUNInvshuman elongation factor 1 alpha (EF-1 alpha) promoter
  • nephronophthisis 2(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman EEF1A1 promoter, mouse Invs cDNA, Jelly fish GFP cDNA
    Research applicationDevelopmental Biology Research
    General Purpose
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 130, 1725-1734 (2003).
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data