Strain Information | |
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Image | |
BRC No. | RBRC01834 |
Type | Transgene![]() |
Species | Mus musculus |
Strain name | C57BL/6-Tg(CAG-flpe)36Ito/ItoRbrc |
Former Common name | B6-Tg(CAG-FLPe)36; C57BL/6-Tg(CAG-FLPe)36Ito/ItoRbrc |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | black [a/a B/B C/C] |
Strain development | 理化学研究所脳科学総合研究センター・神吉浩明先生、糸原重美先生(2006)。C57BL/6受精卵へTgのインジェクションにより作出。C57BL/6J背景。 |
Strain description | CAGプロモーター制御下でFLPeを発現するトランスジェニックマウス。 |
Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
References | High-efficiency CAG-FLPe deleter mice in C57BL/6J background. Kanki H, Suzuki H, Itohara S Exp. Anim., 55, 137-141 (2006). 16651697 |
Health Report | |
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Examination Date / Room / Rack | 2024/11/18Room:4-ARack:F 2024/08/19Room:4-ARack:F 2024/05/20Room:4-ARack:F 2024/02/19Room:4-ARack:F 2023/11/20Room:4-ARack:F 2023/08/28Room:4-ARack:F 2023/08/21Room:4-ARack:F 2023/06/19Room:4-ARack:F 2023/05/22Room:4-ARack:F 2023/02/20Room:4-ARack:F |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
FLPe | yeast flippase recombinase | UN | FLPe | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
IRES | internal ribosomal entry site (EMCV) | UN | |||||
puro | puromycin acyltransferase (Streptomyces alboniger) | UN | puro |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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供与核酸 | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), yeast FLPe cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES), Streptomyces alboniger puromycin resistance gene |
Research application | FLP/frt system General Purpose Genetics Research |
提供条件 | 研究成果の公表にあたって寄託者の指定する文献を引用する。Exp. Anim., 55, 137-141 (2006). |
Depositor | 糸原 重美(独立行政法人理化学研究所) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 生体マウスを3~6ヶ月以内に提供可能 |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Feb 2007 |
BRC mice in Publications |
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Fujinuma S, Nakatsumi H, Shimizu H, Sugiyama S, Harada A, Goya T, Tanaka M, Kohjima M, Takahashi M, Izumi Y, Yagi M, Kang D, Kaneko M, Shigeta M, Bamba T, Ohkawa Y, Nakayama KI. FOXK1 promotes nonalcoholic fatty liver disease by mediating mTORC1-dependent inhibition of hepatic fatty acid oxidation. Cell Rep 42(5) 112530(2023) 37209098 |
Takubo K, Htun PW, Ueda T, Sera Y, Iwasaki M, Koizumi M, Shiroshita K, Kobayashi H, Haraguchi M, Watanuki S, Honda ZI, Yamasaki N, Nakamura-Ishizu A, Arai F, Motoyama N, Hatta T, Natsume T, Suda T, Honda H. MBTD1 preserves adult hematopoietic stem cell pool size and function. Proc Natl Acad Sci U S A 120(32) e2206860120(2023) 37523546 |
Sasaki L, Hamada Y, Yarimizu D, Suzuki T, Nakamura H, Shimada A, Pham KTN, Shao X, Yamamura K, Inatomi T, Morinaga H, Nishimura EK, Kudo F, Manabe I, Haraguchi S, Sugiura Y, Suematsu M, Kinoshita S, Machida M, Nakajima T, Kiyonari H, Okamura H, Yamaguchi Y, Miyake T, Doi M. Intracrine activity involving NAD-dependent circadian steroidogenic activity governs age-associated meibomian gland dysfunction. Nat Aging 2(2) 105-114(2022) 37117756 |
Sera Y, Nakata Y, Ueda T, Yamasaki N, Koide S, Kobayashi H, Ikeda KI, Kobatake K, Iwasaki M, Oda H, Wolff L, Kanai A, Nagamachi A, Inaba T, Sotomaru Y, Ichinohe T, Koizumi M, Miyakawa Y, Honda ZI, Iwama A, Suda T, Takubo K, Honda H. UTX maintains the functional integrity of the murine hematopoietic system by globally regulating aging-associated genes. Blood 137(7) 908-922(2021) 33174606 |
Koga Y, Tsuchimoto D, Hayashi Y, Abolhassani N, Yoneshima Y, Sakumi K, Nakanishi H, Toyokuni S, Nakabeppu Y. Neural stem cell-specific ITPA deficiency causes neural depolarization and epilepsy. JCI Insight 5(22) (2020) 33208550 |
Wada Y, Kikuchi A, Kaga A, Shimizu N, Ito J, Onuma R, Fujishima F, Totsune E, Sato R, Niihori T, Shirota M, Funayama R, Sato K, Nakazawa T, Nakayama K, Aoki Y, Aiba S, Nakagawa K, Kure S. Metabolic and pathologic profiles of human LSS deficiency recapitulated in mice. PLoS Genet 16(2) e1008628(2020) 32101538 |
Kawara A, Mizuta R, Fujisawa M, Ito T, Li C, Nakamura K, Sun C, Kuwabara M, Kitabatake M, Yoshimura T, Matsukawa A. Spred2-deficiency enhances the proliferation of lung epithelial cells and alleviates pulmonary fibrosis induced by bleomycin. Sci Rep 10(1) 16490(2020) 33020583 |
Sakata D, Uruno T, Matsubara K, Andoh T, Yamamura K, Magoshi Y, Kunimura K, Kamikaseda Y, Furue M, Fukui Y. Selective role of neurokinin B in IL-31-induced itch response in mice. J Allergy Clin Immunol 144(4) 1130-1133.e8(2019) 31405606 |
Inoue R, Abdou K, Hayashi-Tanaka A, Muramatsu SI, Mino K, Inokuchi K, Mori H. Glucocorticoid receptor-mediated amygdalar metaplasticity underlies adaptive modulation of fear memory by stress. Elife 7 (2018) 29941090 |
Herndler-Brandstetter D, Ishigame H, Shinnakasu R, Plajer V, Stecher C, Zhao J, Lietzenmayer M, Kroehling L, Takumi A, Kometani K, Inoue T, Kluger Y, Kaech SM, Kurosaki T, Okada T, Flavell RA. KLRG1+ Effector CD8+ T Cells Lose KLRG1, Differentiate into All Memory T Cell Lineages, and Convey Enhanced Protective Immunity. Immunity 48(4) 716-729.e8(2018) 29625895 |
Tsunoda T, Doi K, Ishikura S, Luo H, Nishi K, Matsuzaki H, Koyanagi M, Tanaka Y, Okamura T, Shirasawa S. Zfat expression in ZsGreen reporter gene knock‑in mice: Implications for a novel function of Zfat in definitive erythropoiesis. Int J Mol Med 42(5) 2595-2603(2018) 30106088 |
Kiyozumi D, Taniguchi Y, Nakano I, Toga J, Yagi E, Hasuwa H, Ikawa M, Sekiguchi K. Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethality. Life Sci Alliance 1(5) e201800064(2018) 30456378 |
Kimura-Yoshida C, Mochida K, Nakaya MA, Mizutani T, Matsuo I. Cytoplasmic localization of GRHL3 upon epidermal differentiation triggers cell shape change for epithelial morphogenesis. Nat Commun 9(1) 4059(2018) 30283008 |
Kawai Y, Oda A, Kanai Y, Goitsuka R. Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis. PLoS One 13(1) e0190702(2018) 29293683 |
Yanagihara T, Tomino T, Uruno T, Fukui Y. Thymic epithelial cell-specific deletion of Jmjd6 reduces Aire protein expression and exacerbates disease development in a mouse model of autoimmune diabetes. Biochem Biophys Res Commun 489(1) 8-13(2017) 28546003 |
Nakata Y, Ueda T, Nagamachi A, Yamasaki N, Ikeda KI, Sera Y, Takubo K, Kanai A, Oda H, Sanada M, Ogawa S, Tsuji K, Ebihara Y, Wolff L, Honda ZI, Suda T, Inaba T, Honda H. Acquired expression of CblQ367P in mice induces dysplastic myelopoiesis mimicking chronic myelomonocytic leukemia. Blood 129(15) 2148-2160(2017) 28209720 |
Morooka N, Futaki S, Sato-Nishiuchi R, Nishino M, Totani Y, Shimono C, Nakano I, Nakajima H, Mochizuki N, Sekiguchi K. Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling. Circ Res 120(8) 1276-1288(2017) 28179430 |
Ogino H, Hisanaga A, Kohno T, Kondo Y, Okumura K, Kamei T, Sato T, Asahara H, Tsuiji H, Fukata M, Hattori M. Secreted Metalloproteinase ADAMTS-3 Inactivates Reelin. J Neurosci 37(12) 3181-3191(2017) 28213441 |
Ishikawa T, Kitaura Y, Kadota Y, Morishita Y, Ota M, Yamanaka F, Xu M, Ikawa M, Inoue N, Kawano F, Nakai N, Murakami T, Miura S, Hatazawa Y, Kamei Y, Shimomura Y. Muscle-specific deletion of BDK amplifies loss of myofibrillar protein during protein undernutrition. Sci Rep 7 39825(2017) 28051178 |
Sugimoto M, Shimizu Y, Zhao S, Ukon N, Nishijima K, Wakabayashi M, Yoshioka T, Higashino K, Numata Y, Okuda T, Tamaki N, Hanamatsu H, Igarashi Y, Kuge Y. Characterization of the role of sphingomyelin synthase 2 in glucose metabolism in whole-body and peripheral tissues in mice. Biochim Biophys Acta 1861(8 Pt A) 688-702(2016) 27151272 |
Kai T, Tsukamoto Y, Hijiya N, Tokunaga A, Nakada C, Uchida T, Daa T, Iha H, Takahashi M, Nomura T, Sato F, Mimata H, Ikawa M, Seto M, Matsuura K, Moriyama M. Kidney-specific knockout of Sav1 in the mouse promotes hyperproliferation of renal tubular epithelium through suppression of the Hippo pathway. J Pathol 239(1) 97-108(2016) 26913567 |
Ikeda K, Ueda T, Yamasaki N, Nakata Y, Sera Y, Nagamachi A, Miyama T, Kobayashi H, Takubo K, Kanai A, Oda H, Wolff L, Honda Z, Ichinohe T, Matsubara A, Suda T, Inaba T, Honda H. Maintenance of the functional integrity of mouse hematopoiesis by EED and promotion of leukemogenesis by EED haploinsufficiency. Sci Rep 6 29454(2016) 27432459 |
Kawakami E, Tokunaga A, Ozawa M, Sakamoto R, Yoshida N. The histone demethylase Fbxl11/Kdm2a plays an essential role in embryonic development by repressing cell-cycle regulators. Mech Dev 135 31-42(2015) 25463925 |
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Yoshioka K, Oda A, Notsu C, Ohtsuka T, Kawai Y, Suzuki S, Nakamura T, Mabuchi Y, Matsuzaki Y, Goitsuka R. Loss of the Homeodomain Transcription Factor Prep1 Perturbs Adult Hematopoiesis in the Bone Marrow. PLoS One 10(8) e0136107(2015) 26285139 |
Nakahara R, Kawai Y, Oda A, Nishimura M, Murakami A, Azuma T, Kaifu T, Goitsuka R. Generation of a Tlx1(CreER-Venus) knock-in mouse strain for the study of spleen development. Genesis 52(11) 916-23(2014) 25283275 |
Fumoto T, Takeshita S, Ito M, Ikeda K. Physiological functions of osteoblast lineage and T cell-derived RANKL in bone homeostasis. J Bone Miner Res 29(4) 830-42(2014) 24014480 |
Nagamachi A, Nakata Y, Ueda T, Yamasaki N, Ebihara Y, Tsuji K, Honda Z, Takubo K, Suda T, Oda H, Inaba T, Honda H. Acquired deficiency of A20 results in rapid apoptosis, systemic inflammation, and abnormal hematopoietic stem cell function. PLoS One 9(1) e87425(2014) 24498102 |
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Kimura-Ueki M, Oda Y, Oki J, Komi-Kuramochi A, Honda E, Asada M, Suzuki M, Imamura T. Hair cycle resting phase is regulated by cyclic epithelial FGF18 signaling. J Invest Dermatol 132(5) 1338-45(2012) 22297635 |
Maeda K, Kobayashi Y, Udagawa N, Uehara S, Ishihara A, Mizoguchi T, Kikuchi Y, Takada I, Kato S, Kani S, Nishita M, Marumo K, Martin TJ, Minami Y, Takahashi N. Wnt5a-Ror2 signaling between osteoblast-lineage cells and osteoclast precursors enhances osteoclastogenesis. Nat Med 18(3) 405-12(2012) 22344299 |
Hara T, Mimura K, Abe T, Shioi G, Seiki M, Sakamoto T. Deletion of the Mint3/Apba3 gene in mice abrogates macrophage functions and increases resistance to lipopolysaccharide-induced septic shock. J Biol Chem 286(37) 32542-51(2011) 21778228 |
Kobayashi N, Miyoshi S, Mikami T, Koyama H, Kitazawa M, Takeoka M, Sano K, Amano J, Isogai Z, Niida S, Oguri K, Okayama M, McDonald JA, Kimata K, Taniguchi S, Itano N. Hyaluronan deficiency in tumor stroma impairs macrophage trafficking and tumor neovascularization. Cancer Res 70(18) 7073-83(2010) 20823158 |
Nakata H, Hashimoto T, Seki Y, Mekada K, Obata Y, Yoshiki A. Simultaneous detection of multiple transgenes for genetically-modified mouse strains. Exp Anim 58(4) 437-42(2009) 19654444 |