Strain Data Sheet

RBRC01834

Strain Information

Image
BRC No.RBRC01834
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG-flpe)36Ito/ItoRbrc
Former Common nameB6-Tg(CAG-FLPe)36; C57BL/6-Tg(CAG-FLPe)36Ito/ItoRbrc
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain development理化学研究所脳科学総合研究センター・神吉浩明先生、糸原重美先生(2006)。C57BL/6受精卵へTgのインジェクションにより作出。C57BL/6J背景。
Strain descriptionCAGプロモーター制御下でFLPeを発現するトランスジェニックマウス。
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
High-efficiency CAG-FLPe deleter mice in C57BL/6J background.
Kanki H, Suzuki H, Itohara S
Exp. Anim., 55, 137-141 (2006). 16651697

Health Report

Examination Date / Room / Rack2024/11/18Room:4-ARack:F
2024/08/19Room:4-ARack:F
2024/05/20Room:4-ARack:F
2024/02/19Room:4-ARack:F
2023/11/20Room:4-ARack:F
2023/08/28Room:4-ARack:F
2023/08/21Room:4-ARack:F
2023/06/19Room:4-ARack:F
2023/05/22Room:4-ARack:F
2023/02/20Room:4-ARack:F

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FLPe yeast flippase recombinaseUNFLPe CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
IRES internal ribosomal entry site (EMCV)UN
puro puromycin acyltransferase (Streptomyces alboniger)UNpuro

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
  • Detailed phenotype data

    Ordering Information

    供与核酸CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), yeast FLPe cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES), Streptomyces alboniger puromycin resistance gene
    Research applicationFLP/frt system
    General Purpose
    Genetics Research
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。Exp. Anim., 55, 137-141 (2006).
    Depositor糸原 重美(独立行政法人理化学研究所)
    Strain Status生体のアイコン生体
    凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    生体マウスを3~6ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Feb 2007

    BRC mice in Publications

    Fujinuma S, Nakatsumi H, Shimizu H, Sugiyama S, Harada A, Goya T, Tanaka M, Kohjima M, Takahashi M, Izumi Y, Yagi M, Kang D, Kaneko M, Shigeta M, Bamba T, Ohkawa Y, Nakayama KI.
    FOXK1 promotes nonalcoholic fatty liver disease by mediating mTORC1-dependent inhibition of hepatic fatty acid oxidation.
    Cell Rep 42(5) 112530(2023) 37209098
    Takubo K, Htun PW, Ueda T, Sera Y, Iwasaki M, Koizumi M, Shiroshita K, Kobayashi H, Haraguchi M, Watanuki S, Honda ZI, Yamasaki N, Nakamura-Ishizu A, Arai F, Motoyama N, Hatta T, Natsume T, Suda T, Honda H.
    MBTD1 preserves adult hematopoietic stem cell pool size and function.
    Proc Natl Acad Sci U S A 120(32) e2206860120(2023) 37523546
    Sasaki L, Hamada Y, Yarimizu D, Suzuki T, Nakamura H, Shimada A, Pham KTN, Shao X, Yamamura K, Inatomi T, Morinaga H, Nishimura EK, Kudo F, Manabe I, Haraguchi S, Sugiura Y, Suematsu M, Kinoshita S, Machida M, Nakajima T, Kiyonari H, Okamura H, Yamaguchi Y, Miyake T, Doi M.
    Intracrine activity involving NAD-dependent circadian steroidogenic activity governs age-associated meibomian gland dysfunction.
    Nat Aging 2(2) 105-114(2022) 37117756
    Sera Y, Nakata Y, Ueda T, Yamasaki N, Koide S, Kobayashi H, Ikeda KI, Kobatake K, Iwasaki M, Oda H, Wolff L, Kanai A, Nagamachi A, Inaba T, Sotomaru Y, Ichinohe T, Koizumi M, Miyakawa Y, Honda ZI, Iwama A, Suda T, Takubo K, Honda H.
    UTX maintains the functional integrity of the murine hematopoietic system by globally regulating aging-associated genes.
    Blood 137(7) 908-922(2021) 33174606
    Koga Y, Tsuchimoto D, Hayashi Y, Abolhassani N, Yoneshima Y, Sakumi K, Nakanishi H, Toyokuni S, Nakabeppu Y.
    Neural stem cell-specific ITPA deficiency causes neural depolarization and epilepsy.
    JCI Insight 5(22) (2020) 33208550
    Wada Y, Kikuchi A, Kaga A, Shimizu N, Ito J, Onuma R, Fujishima F, Totsune E, Sato R, Niihori T, Shirota M, Funayama R, Sato K, Nakazawa T, Nakayama K, Aoki Y, Aiba S, Nakagawa K, Kure S.
    Metabolic and pathologic profiles of human LSS deficiency recapitulated in mice.
    PLoS Genet 16(2) e1008628(2020) 32101538
    Kawara A, Mizuta R, Fujisawa M, Ito T, Li C, Nakamura K, Sun C, Kuwabara M, Kitabatake M, Yoshimura T, Matsukawa A.
    Spred2-deficiency enhances the proliferation of lung epithelial cells and alleviates pulmonary fibrosis induced by bleomycin.
    Sci Rep 10(1) 16490(2020) 33020583
    Sakata D, Uruno T, Matsubara K, Andoh T, Yamamura K, Magoshi Y, Kunimura K, Kamikaseda Y, Furue M, Fukui Y.
    Selective role of neurokinin B in IL-31-induced itch response in mice.
    J Allergy Clin Immunol 144(4) 1130-1133.e8(2019) 31405606
    Inoue R, Abdou K, Hayashi-Tanaka A, Muramatsu SI, Mino K, Inokuchi K, Mori H.
    Glucocorticoid receptor-mediated amygdalar metaplasticity underlies adaptive modulation of fear memory by stress.
    Elife 7 (2018) 29941090
    Herndler-Brandstetter D, Ishigame H, Shinnakasu R, Plajer V, Stecher C, Zhao J, Lietzenmayer M, Kroehling L, Takumi A, Kometani K, Inoue T, Kluger Y, Kaech SM, Kurosaki T, Okada T, Flavell RA.
    KLRG1+ Effector CD8+ T Cells Lose KLRG1, Differentiate into All Memory T Cell Lineages, and Convey Enhanced Protective Immunity.
    Immunity 48(4) 716-729.e8(2018) 29625895
    Tsunoda T, Doi K, Ishikura S, Luo H, Nishi K, Matsuzaki H, Koyanagi M, Tanaka Y, Okamura T, Shirasawa S.
    Zfat expression in ZsGreen reporter gene knock‑in mice: Implications for a novel function of Zfat in definitive erythropoiesis.
    Int J Mol Med 42(5) 2595-2603(2018) 30106088
    Kiyozumi D, Taniguchi Y, Nakano I, Toga J, Yagi E, Hasuwa H, Ikawa M, Sekiguchi K.
    Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethality.
    Life Sci Alliance 1(5) e201800064(2018) 30456378
    Kimura-Yoshida C, Mochida K, Nakaya MA, Mizutani T, Matsuo I.
    Cytoplasmic localization of GRHL3 upon epidermal differentiation triggers cell shape change for epithelial morphogenesis.
    Nat Commun 9(1) 4059(2018) 30283008
    Kawai Y, Oda A, Kanai Y, Goitsuka R.
    Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis.
    PLoS One 13(1) e0190702(2018) 29293683
    Yanagihara T, Tomino T, Uruno T, Fukui Y.
    Thymic epithelial cell-specific deletion of Jmjd6 reduces Aire protein expression and exacerbates disease development in a mouse model of autoimmune diabetes.
    Biochem Biophys Res Commun 489(1) 8-13(2017) 28546003
    Nakata Y, Ueda T, Nagamachi A, Yamasaki N, Ikeda KI, Sera Y, Takubo K, Kanai A, Oda H, Sanada M, Ogawa S, Tsuji K, Ebihara Y, Wolff L, Honda ZI, Suda T, Inaba T, Honda H.
    Acquired expression of CblQ367P in mice induces dysplastic myelopoiesis mimicking chronic myelomonocytic leukemia.
    Blood 129(15) 2148-2160(2017) 28209720
    Morooka N, Futaki S, Sato-Nishiuchi R, Nishino M, Totani Y, Shimono C, Nakano I, Nakajima H, Mochizuki N, Sekiguchi K.
    Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling.
    Circ Res 120(8) 1276-1288(2017) 28179430
    Ogino H, Hisanaga A, Kohno T, Kondo Y, Okumura K, Kamei T, Sato T, Asahara H, Tsuiji H, Fukata M, Hattori M.
    Secreted Metalloproteinase ADAMTS-3 Inactivates Reelin.
    J Neurosci 37(12) 3181-3191(2017) 28213441
    Ishikawa T, Kitaura Y, Kadota Y, Morishita Y, Ota M, Yamanaka F, Xu M, Ikawa M, Inoue N, Kawano F, Nakai N, Murakami T, Miura S, Hatazawa Y, Kamei Y, Shimomura Y.
    Muscle-specific deletion of BDK amplifies loss of myofibrillar protein during protein undernutrition.
    Sci Rep 7 39825(2017) 28051178
    Sugimoto M, Shimizu Y, Zhao S, Ukon N, Nishijima K, Wakabayashi M, Yoshioka T, Higashino K, Numata Y, Okuda T, Tamaki N, Hanamatsu H, Igarashi Y, Kuge Y.
    Characterization of the role of sphingomyelin synthase 2 in glucose metabolism in whole-body and peripheral tissues in mice.
    Biochim Biophys Acta 1861(8 Pt A) 688-702(2016) 27151272
    Kai T, Tsukamoto Y, Hijiya N, Tokunaga A, Nakada C, Uchida T, Daa T, Iha H, Takahashi M, Nomura T, Sato F, Mimata H, Ikawa M, Seto M, Matsuura K, Moriyama M.
    Kidney-specific knockout of Sav1 in the mouse promotes hyperproliferation of renal tubular epithelium through suppression of the Hippo pathway.
    J Pathol 239(1) 97-108(2016) 26913567
    Ikeda K, Ueda T, Yamasaki N, Nakata Y, Sera Y, Nagamachi A, Miyama T, Kobayashi H, Takubo K, Kanai A, Oda H, Wolff L, Honda Z, Ichinohe T, Matsubara A, Suda T, Inaba T, Honda H.
    Maintenance of the functional integrity of mouse hematopoiesis by EED and promotion of leukemogenesis by EED haploinsufficiency.
    Sci Rep 6 29454(2016) 27432459
    Kawakami E, Tokunaga A, Ozawa M, Sakamoto R, Yoshida N.
    The histone demethylase Fbxl11/Kdm2a plays an essential role in embryonic development by repressing cell-cycle regulators.
    Mech Dev 135 31-42(2015) 25463925
    Raveney BJ, Oki S, Hohjoh H, Nakamura M, Sato W, Murata M, Yamamura T.
    Eomesodermin-expressing T-helper cells are essential for chronic neuroinflammation.
    Nat Commun 6 8437(2015) 26436530
    Yoshioka K, Oda A, Notsu C, Ohtsuka T, Kawai Y, Suzuki S, Nakamura T, Mabuchi Y, Matsuzaki Y, Goitsuka R.
    Loss of the Homeodomain Transcription Factor Prep1 Perturbs Adult Hematopoiesis in the Bone Marrow.
    PLoS One 10(8) e0136107(2015) 26285139
    Nakahara R, Kawai Y, Oda A, Nishimura M, Murakami A, Azuma T, Kaifu T, Goitsuka R.
    Generation of a Tlx1(CreER-Venus) knock-in mouse strain for the study of spleen development.
    Genesis 52(11) 916-23(2014) 25283275
    Fumoto T, Takeshita S, Ito M, Ikeda K.
    Physiological functions of osteoblast lineage and T cell-derived RANKL in bone homeostasis.
    J Bone Miner Res 29(4) 830-42(2014) 24014480
    Nagamachi A, Nakata Y, Ueda T, Yamasaki N, Ebihara Y, Tsuji K, Honda Z, Takubo K, Suda T, Oda H, Inaba T, Honda H.
    Acquired deficiency of A20 results in rapid apoptosis, systemic inflammation, and abnormal hematopoietic stem cell function.
    PLoS One 9(1) e87425(2014) 24498102
    Okuma A, Hoshino K, Ohba T, Fukushi S, Aiba S, Akira S, Ono M, Kaisho T, Muta T.
    Enhanced apoptosis by disruption of the STAT3-IκB-ζ signaling pathway in epithelial cells induces Sjögren's syndrome-like autoimmune disease.
    Immunity 38(3) 450-60(2013) 23453632
    Kaji T, Ishige A, Hikida M, Taka J, Hijikata A, Kubo M, Nagashima T, Takahashi Y, Kurosaki T, Okada M, Ohara O, Rajewsky K, Takemori T.
    Distinct cellular pathways select germline-encoded and somatically mutated antibodies into immunological memory.
    J Exp Med 209(11) 2079-97(2012) 23027924
    Kimura-Ueki M, Oda Y, Oki J, Komi-Kuramochi A, Honda E, Asada M, Suzuki M, Imamura T.
    Hair cycle resting phase is regulated by cyclic epithelial FGF18 signaling.
    J Invest Dermatol 132(5) 1338-45(2012) 22297635
    Maeda K, Kobayashi Y, Udagawa N, Uehara S, Ishihara A, Mizoguchi T, Kikuchi Y, Takada I, Kato S, Kani S, Nishita M, Marumo K, Martin TJ, Minami Y, Takahashi N.
    Wnt5a-Ror2 signaling between osteoblast-lineage cells and osteoclast precursors enhances osteoclastogenesis.
    Nat Med 18(3) 405-12(2012) 22344299
    Hara T, Mimura K, Abe T, Shioi G, Seiki M, Sakamoto T.
    Deletion of the Mint3/Apba3 gene in mice abrogates macrophage functions and increases resistance to lipopolysaccharide-induced septic shock.
    J Biol Chem 286(37) 32542-51(2011) 21778228
    Kobayashi N, Miyoshi S, Mikami T, Koyama H, Kitazawa M, Takeoka M, Sano K, Amano J, Isogai Z, Niida S, Oguri K, Okayama M, McDonald JA, Kimata K, Taniguchi S, Itano N.
    Hyaluronan deficiency in tumor stroma impairs macrophage trafficking and tumor neovascularization.
    Cancer Res 70(18) 7073-83(2010) 20823158
    Nakata H, Hashimoto T, Seki Y, Mekada K, Obata Y, Yoshiki A.
    Simultaneous detection of multiple transgenes for genetically-modified mouse strains.
    Exp Anim 58(4) 437-42(2009) 19654444