Strain Data Sheet

RBRC01737

Strain Information

Image
BRC No.RBRC01737
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Dnmt1<tm2Enl>
Former Common nameDnmt<1c>, Dnmt<c>, Dnmt1<tm2Enl>
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentEn Li, Massachusetts General HospitalMassachusetts General Hospitalで樹立理研CDBでの世代数３～５代
Strain description1.ホモマウス胚は胎生9.5日で致死2.胎生8.0-8.5日 (体節0～5個) で発生停止3.解析した限り、ほぼ全ての遺伝子、反復配列において、ゲノムDNAメチル化の顕著な低下が認められる4.ヘテロマウスは正常に発生・交配可能
Colony maintenanceヘテロで交配維持ホモで不妊／死亡
ReferencesDevelopment, 122, 3195-3205 (1996). 8898232

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dnmt1DNA methyltransferase (cytosine-5) 19Dnmt1<tm2Enl>targeted mutation 2, En LiCxxc9, Dnmt1o, MTase

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)9neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

供与核酸mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Pgk1 polyA, mouse Dnmt1 genomic DNA
Research application
提供条件条件を付加する。
A. RECIPIENT must send a copy of the executed MTA between RECIPIENT and RIKEN BRC to: Massachusetts General Hospital c/o Partners Innovation, Attn: TAG/MGH MTA 2020A005592, 399 Revolution Drive/Ste 950E, Somerville,MA 02245, USA (phsmta@partners.org). B. Any publication or public disclosure of research results obtained by the use of the BIOLOGICAL RESOURCE shall cite Massachusetts General Hospital in Boston, MA as the source of the material. However, neither the name, trademark, service mark, logo nor other identifying characteristic ("Name") of MGH or any of its affiliates, or any of its or their respective directors, trustees, officers, appointees, employees, staff, representatives or agents, in any advertising, promotional or sales literature, publicity or in any document employed to obtain funds or financing without the prior written approval of the MGH Department of Public Affairs. C. In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature Development 1996 122:3195-3205 as designated by the DEPOSITOR is required. D. The use of the BIOLOGICAL RESOURCE is restricted to academic researchers in non-profit organizations for their internal research and educational purposes. E. The BIOLOGICAL RESOURCE shall not be used for commercial purposes. Any request for the BIOLOGICAL RESOURCE by a for-profit entity shall be referred to Massachusetts General Hospital through the Research and Licensing Office. F. Recipients shall assume all liability for their use, storage, handling and disposal of the BIOLOGICAL RESOURCE. The General Hospital Corporation d/b/a Massachusetts General Hospital will not be liable to the Recipients for any loss, claims, matters, damages, costs or liabilities relating to any third party actions, proceedings, investigations, or matters arising from any use, storage, handling, or disposal of the BIOLOGICAL RESOURCE by Recipient.
Depositor岡野　正樹（独立行政法人理化学研究所とMGH）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Please use the MATERIAL TRANSFER AGREEMENT for Distribution for non-profit or for profit (English only).
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data