Strain Data Sheet

RBRC01733

Strain Information

Image
BRC No.RBRC01733
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Ptafr<tm1Tksh>/TkshRbrc
Former Common namePAFR-KO, Platelet-activating factor (PAF) receptor knockout mouse
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6NCrlCrlj
Appearanceblack [a/a B/B C/C]
Strain developmentC57BL/6 x 129/Olaキメラから生まれたマウスをC57BL/6と9回交配しました（N10）
Strain descriptionPlatelet-activating factor (PAF) is a phospholipid mediator with pleiotropic and potent biological effects on a variety of cells and tissues. Homozygous mutant mice showed decreased susceptibility to bacterial infection. This strain is useful to elucidating the pathophysiological roles of PAF and PAFR in vivo.
Colony maintenanceB6との戻し交配が進むにつれKOどうしの交配による繁殖効率が悪くなっている。ただし全く繁殖しないわけではない。現在、ヘテロ同士で交配。繁殖成績が低い
ReferencesJ. Exp. Med., 187, 1779-1788 (1998). 9607919

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ptafrplatelet-activating factor receptor4Ptafr<tm1Tksh>targeted mutation 1, Takao ShimizuPAF receptor, PAFR

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

供与核酸mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Pgk polyA, mouse platelet-activating factor receptor (PAF) genomic DNA
Research application
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。J. Exp. Med., 187, 1779-1788 (1998).
Depositor清水　孝雄（国立大学法人東京大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

Tanaka Y, Nagai Y, Kuroishi T, Endo Y, Sugawara S.
Stimulation of Ly-6G on neutrophils in LPS-primed mice induces platelet-activating factor (PAF)-mediated anaphylaxis-like shock.
J Leukoc Biol 91(3) 485-94(2012) 22131343

Kim BK, Shin EJ, Kim HC, Chung YH, Dang DK, Jung BD, Park DH, Wie MB, Kim WK, Shimizu T, Nabeshima T, Jeong JH.
Platelet-activating factor receptor knockout mice are protected from MPTP-induced dopaminergic degeneration.
Neurochem Int 63(3) 121-32(2013) 23743065