Strain Information | |
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Image | |
BRC No. | RBRC01726 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129-Mfge8<tm1Osa>/OsaRbrc |
Former Common name | MFG-E8 KO; MFG-E8 Knockout mouse |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | C57BL/6 |
Appearance | |
Strain development | Developed by Drs. Rikinari Hanayama and Shigekazu Nagata at Osaka University in 2004. A neomycin cassette replaced exons 4-6 of MFGE-8 gene. The mutant mice were backcrossed to C57BL/6N for at least 9 times. |
Strain description | B6;129-Mfge8<tm1Osa>. Apoptotic cells are rapidly uptaken by macrophages. This process is essential to prevent inflammation and the autoimmune response against intracellular antigens released from dying cells. Milk fat globule epidermal growth factor 8 (MFG-E8) is a protein that specifically binds to apoptotic cells and enhance the uptake of apoptotic cells by macrophages. The MFG-E8 deficient mice developed splenomegaly and suffered from glomerulonephritis as a result of autoantibody production. MFG-E8 is considered to have a critical role in removing apoptotic B cells in spleen and lymph nodes, and its deficiency can lead to autoimmune disease. |
Colony maintenance | Homozygote x Homozygote |
References | Autoimmune disease and impaired uptake of apoptotic cells in MFG-E8-deficient mice. Hanayama R, Tanaka M, Miyasaka K, Aozasa K, Koike M, Uchiyama Y, Nagata S Science, 304, 1147-1150 (2004). 15155946 |
Health Report | |
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Examination Date / Room / Rack | 2024/10/07Room:3-BRack:A 2024/07/08Room:3-BRack:A 2024/04/08Room:3-BRack:A 2024/01/09Room:3-BRack:A 2023/10/10Room:3-BRack:A 2023/07/10Room:3-BRack:A 2023/04/10Room:3-BRack:A 2023/01/10Room:3-BRack:A 2022/12/19Room:3-BRack:A |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Mfge8 | milk fat globule EGF and factor V/VIII domain containing | 7 | Mfge8 | targeted mutation 1, Shigekazu Nagata | |||
neo | neomycin resistance gene (E. coli) | 7 | mouse phosphoglycerate kinase promoter (PGK promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 9 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | mouse phosphoglycerate kinase-1 promoter, E. coli neo, mouse MFG-E8 genomic DNA |
Research application | Immunology and Inflammation Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 304, 1147-1150 (2004). RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose. The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. For period of two (2) years after deposition by the DEPOSITOR to the REKEN BRC, the RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author. |
Depositor | Shigekazu Nagata (Kyoto University) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Nov 2006 |
BRC mice in Publications |
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Chiang HY, Chu PH, Chen SC, Lee TH. MFG-E8 promotes osteogenic transdifferentiation of smooth muscle cells and vascular calcification by regulating TGF-β1 signaling. Commun Biol 5(1) 364(2022) 35440618 |