Strain Data Sheet

RBRC01724

Strain Information

Image
BRC No.RBRC01724
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Dnase2b<tm1Osa>/OsaRbrc
Former Common nameDLAD KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Nuclear cataract caused by a lack of DNA degradation in the mouse eye lens.
Nishimoto S, Kawane K, Watanabe-Fukunaga R, Fukuyama H, Ohsawa Y, Uchiyama Y, Hashida N, Ohguro N, Tano Y, Morimoto T, Fukuda Y, Nagata S
Nature, 424, 1071-1074 (2003). 12944971

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dnase2bdeoxyribonuclease II beta3Dnase2btargeted mutation 1, Shigekazu Nagata
neoneomycin resistance gene (E. coli)3mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal double-strand DNA break repair(MP:0010948)

  • abnormal endoplasmic reticulum morphology(MP:0009378)

  • abnormal eye electrophysiology(MP:0005551)

  • abnormal vision(MP:0002090)

  • cataract(MP:0001304)
  • more 1 phenotypes
  • decreased mitochondrial number(MP:0011629)
  • Detailed phenotype data

    Ordering Information

    Donor DNAPGK-NeoR 遺伝子(Mouse phosphoglycerate kinase-1 promoter, E. coli ネオマイシン耐性遺伝子), DLAD 遺伝子
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 424, 1071-1074 (2003).RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
    The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    For period of two (2) years after deposition by the DEPOSITOR to the REKEN BRC, the RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author.
    DepositorShigekazu Nagata (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data